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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- PA5-31695 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NSUN4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HepG2, HCT116, mouse liver, rat liver.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NSUN4 was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a NSUN4 Polyclonal Antibody (Product # PA5-31695) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NSUN4 Polyclonal Antibody (Product # PA5-31695). Sample (30 µg of whole cell lysate). Lane A: Hep G2. 10% SDS PAGE. NSUN4 Polyclonal Antibody (Product # PA5-31695) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NSUN4 Polyclonal Antibody (Product # PA5-31695). Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSUN4 Polyclonal Antibody (Product # PA5-31695) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NSUN4 was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a NSUN4 Polyclonal Antibody (Product # PA5-31695) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NSUN4 (Product # PA5-31695) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
