H00008569-M05

antibody from Abnova Corporation

Targeting: MKNK1 MNK1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00008569-M05

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00008569-M05, RRID:AB_534941

Product name

MKNK1 monoclonal antibody (M05), clone 5E2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant MKNK1.

Antigen sequence

MVSSQKLEKPIEMGSSEPLPIADGDRRRKKKRRGR
ATDSLPGKFEDMYKLTSELLGEGAYAKVQGAVSLQ
NGKEYAVKIIEKQAGHSRSRVFREVETLYQCQRNK
NILELIEFFEDDTRFYLVFEKLQGGSILAHIQKQK
HFNEREASRVVRGVAAALDFLHTKDKVSLCHLGWS
AMAPSGLTAAPTSLGSSDPPTSASQVAGTTGIAHR
DLKPENILCESPEKVSPVKICDFDLGSGMKLNNSC
TPITTPELTTPCGSAEYMAPEVVEVFTDQATFYDK
RCDLWSLGVVLYIMLSGYPPFVGHCGADCGWDRGE
VCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDL
ISKLLVRDAKQRLSAAQVLQHPWVQGQAPEKGLPT
PQVLQRNSSTMDLTLFAAEAIALNRLLSQHEENEL
AEEPEALADGLCSMKLSPPCKSRLARRRALAQAGR
GEDRSPPTAL


Isotype

IgG

Antibody clone number

5E2

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MKNK1 expression in transfected 293T cell line by MKNK1 monoclonal antibody (M05), clone 5E2.Lane 1: MKNK1 transfected lysate(51.3 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged MKNK1 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to MKNK1 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol