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- References [13]
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- Product number
- M03989-3 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Tubulin alpha Picoband™ Antibody (monoclonal, 7B12)
- Antibody type
- Monoclonal
- Description
- Mouse IgG monoclonal antibody for Tubulin alpha detection. Tested with WB, IHC-P, ICC/IF, FCM in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 7B12
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references The anti-HIV drug abacavir stimulates β-catenin activity in osteoblast lineage cells.
Protein nanoparticle-induced osmotic pressure gradients modify pulmonary edema through hyperpermeability in acute respiratory distress syndrome.
Protein Nanoparticle-Related Osmotic Pressure Modifies Nonselective Permeability of the Blood-Brain Barrier by Increasing Membrane Fluidity.
Baicalein inhibits non-small-cell lung cancer invasion and metastasis by reducing ezrin tension in inflammation microenvironment.
Design, synthesis and antineoplastic activity of novel 20(S)-acylthiourea derivatives of camptothecin.
Regulation of ezrin tension by S-nitrosylation mediates non-small cell lung cancer invasion and metastasis.
Participation of tumor suppressors long non-coding RNA MEG3, microRNA-377 and PTEN in glioma cell invasion and migration.
Polydatin attenuates diet-induced nonalcoholic steatohepatitis and fibrosis in mice.
Fasudil inhibits proliferation and migration of Hep-2 laryngeal carcinoma cells.
Early growth response-1 negative feedback regulates skeletal muscle postprandial insulin sensitivity via activating Ptp1b transcription.
Stably maintained microtubules protect dopamine neurons and alleviate depression-like behavior after intracerebral hemorrhage.
Attenuation of TGFBR2 expression and tumour progression in prostate cancer involve diverse hypoxia-regulated pathways.
A proteomic view of Caenorhabditis elegans caused by short-term hypoxic stress.
Olali AZ, Wallace J, Gonzalez H, Carpenter KA, Patel N, Winchester LC, Podany AT, Venkatesh I, Narasipura SD, Al-Harthi L, Ross RD
JBMR plus 2024 May;8(5):ziae037
JBMR plus 2024 May;8(5):ziae037
Protein nanoparticle-induced osmotic pressure gradients modify pulmonary edema through hyperpermeability in acute respiratory distress syndrome.
Qian Z, Wang Q, Qiu Z, Li D, Zhang C, Xiong X, Zheng Z, Ruan Q, Guo Y, Guo J
Journal of nanobiotechnology 2022 Jul 6;20(1):314
Journal of nanobiotechnology 2022 Jul 6;20(1):314
Protein Nanoparticle-Related Osmotic Pressure Modifies Nonselective Permeability of the Blood-Brain Barrier by Increasing Membrane Fluidity.
Li C, Chen L, Wang Y, Wang T, Di D, Zhang H, Zhao H, Shen X, Guo J
International journal of nanomedicine 2021;16:1663-1680
International journal of nanomedicine 2021;16:1663-1680
Baicalein inhibits non-small-cell lung cancer invasion and metastasis by reducing ezrin tension in inflammation microenvironment.
Zhang X, Ruan Q, Zhai Y, Lu D, Li C, Fu Y, Zheng Z, Song Y, Guo J
Cancer science 2020 Oct;111(10):3802-3812
Cancer science 2020 Oct;111(10):3802-3812
Design, synthesis and antineoplastic activity of novel 20(S)-acylthiourea derivatives of camptothecin.
Yang CJ, Li B, Zhang ZJ, Gao JM, Wang MJ, Zhao XB, Song ZL, Liu YQ, Li H, Chen Y, Lee KH, Morris-Natschke SL, Xu C
European journal of medicinal chemistry 2020 Feb 1;187:111971
European journal of medicinal chemistry 2020 Feb 1;187:111971
Regulation of ezrin tension by S-nitrosylation mediates non-small cell lung cancer invasion and metastasis.
Zhang X, Li G, Guo Y, Song Y, Chen L, Ruan Q, Wang Y, Sun L, Hu Y, Zhou J, Ren B, Guo J
Theranostics 2019;9(9):2555-2571
Theranostics 2019;9(9):2555-2571
Participation of tumor suppressors long non-coding RNA MEG3, microRNA-377 and PTEN in glioma cell invasion and migration.
Wang D, Fu CW, Fan DQ
Pathology, research and practice 2019 Oct;215(10):152558
Pathology, research and practice 2019 Oct;215(10):152558
Polydatin attenuates diet-induced nonalcoholic steatohepatitis and fibrosis in mice.
Li R, Li J, Huang Y, Li H, Yan S, Lin J, Chen Y, Wu L, Liu B, Wang G, Lan T
International journal of biological sciences 2018;14(11):1411-1425
International journal of biological sciences 2018;14(11):1411-1425
Fasudil inhibits proliferation and migration of Hep-2 laryngeal carcinoma cells.
Zhang X, Wu N
Drug design, development and therapy 2018;12:373-381
Drug design, development and therapy 2018;12:373-381
Early growth response-1 negative feedback regulates skeletal muscle postprandial insulin sensitivity via activating Ptp1b transcription.
Wu J, Tao WW, Chong DY, Lai SS, Wang C, Liu Q, Zhang TY, Xue B, Li CJ
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Aug;32(8):4370-4379
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Aug;32(8):4370-4379
Stably maintained microtubules protect dopamine neurons and alleviate depression-like behavior after intracerebral hemorrhage.
Yang Y, Zhang K, Zhong J, Wang J, Yu Z, Lei X, Chen X, Quan Y, Xian J, Chen Y, Liu X, Feng H, Tan L
Scientific reports 2018 Aug 23;8(1):12647
Scientific reports 2018 Aug 23;8(1):12647
Attenuation of TGFBR2 expression and tumour progression in prostate cancer involve diverse hypoxia-regulated pathways.
Zhou H, Wu G, Ma X, Xiao J, Yu G, Yang C, Xu N, Zhang B, Zhou J, Ye Z, Wang Z
Journal of experimental & clinical cancer research : CR 2018 Apr 27;37(1):89
Journal of experimental & clinical cancer research : CR 2018 Apr 27;37(1):89
A proteomic view of Caenorhabditis elegans caused by short-term hypoxic stress.
Li H, Ren C, Shi J, Hang X, Zhang F, Gao Y, Wu Y, Xu L, Chen C, Zhang C
Proteome science 2010 Sep 21;8:49
Proteome science 2010 Sep 21;8:49
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of Tubulin alpha using anti-Tubulin alpha antibody (M03989-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Tubulin alpha antigen affinity purified monoclonal antibody (Catalog # M03989-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Tubulin alpha at approximately 56KD. The expected band size for Tubulin alpha is at 56KD.
- Additional image
