Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF399 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Annexin V Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse and rat Annexin V in Western blots. Detects human Annexin V in direct ELISAs.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P08758- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Megf10 Is a Receptor for C1Q That Mediates Clearance of Apoptotic Cells by Astrocytes.
The tolerogenic function of annexins on apoptotic cells is mediated by the annexin core domain.
Type II collagen levels correlate with mineralization by articular cartilage vesicles.
Proteomic assessment of sympathetic ganglia from adult mice that possess null mutations of ExonIII or ExonIV in the p75 neurotrophin receptor gene.
Iram T, Ramirez-Ortiz Z, Byrne MH, Coleman UA, Kingery ND, Means TK, Frenkel D, El Khoury J
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 May 11;36(19):5185-92
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 May 11;36(19):5185-92
The tolerogenic function of annexins on apoptotic cells is mediated by the annexin core domain.
Linke B, Abeler-Dörner L, Jahndel V, Kurz A, Mahr A, Pfrang S, Linke L, Krammer PH, Weyd H
Journal of immunology (Baltimore, Md. : 1950) 2015 Jun 1;194(11):5233-42
Journal of immunology (Baltimore, Md. : 1950) 2015 Jun 1;194(11):5233-42
Type II collagen levels correlate with mineralization by articular cartilage vesicles.
Jubeck B, Muth E, Gohr CM, Rosenthal AK
Arthritis and rheumatism 2009 Sep;60(9):2741-6
Arthritis and rheumatism 2009 Sep;60(9):2741-6
Proteomic assessment of sympathetic ganglia from adult mice that possess null mutations of ExonIII or ExonIV in the p75 neurotrophin receptor gene.
McDonald TG, Scott SA, Kane KM, Kawaja MD
Brain research 2009 Feb 9;1253:1-14
Brain research 2009 Feb 9;1253:1-14
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat Annexin V by Western Blot. Western blot shows lysates of human lung tissue, mouse lung tissue, and rat lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Annexin V Antigen Affinity-purified Polyclonal Antibody (Catalog # AF399) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Annexin V at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
