- GTX100446 - GeneTex CDKN1B antibody

Submitted references Melatonin Inhibits in Vitro Smooth Muscle Cell Inflammation and Proliferation and Atherosclerosis in Apolipoprotein E-Deficient Mice.

Li HY, Leu YL, Wu YC, Wang SH
Journal of agricultural and food chemistry 2019 Feb 20;67(7):1889-1901

A high-throughput pipeline for validation of antibodies.

Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912

Suppression of Cell Growth, Migration and Drug Resistance by Ethanolic Extract of Antrodia cinnamomea in Human Lung Cancer A549 Cells and C57BL/6J Allograft Tumor Model.

Wu CH, Liu FC, Pan CH, Lai MT, Lan SJ, Wu CH, Sheu MJ
International journal of molecular sciences 2018 Mar 9;19(3)

DNA damage induced by topoisomerase inhibitors activates SAMHD1 and blocks HIV-1 infection of macrophages.

Mlcochova P, Caswell SJ, Taylor IA, Towers GJ, Gupta RK
The EMBO journal 2018 Jan 4;37(1):50-62

Honokiol, a potential therapeutic agent, induces cell cycle arrest and program cell death in vitro and in vivo in human thyroid cancer cells.

Lu CH, Chen SH, Chang YS, Liu YW, Wu JY, Lim YP, Yu HI, Lee YR
Pharmacological research 2017 Jan;115:288-298

Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells.

Liu YH, Weng YP, Lin HY, Tang SW, Chen CJ, Liang CJ, Ku CY, Lin JY
Scientific reports 2017 Jan 30;7:41437

Viscolin Inhibits In Vitro Smooth Muscle Cell Proliferation and Migration and Neointimal Hyperplasia In Vivo.

Chen CC, Liang CJ, Leu YL, Chen YL, Wang SH
PloS one 2016;11(12):e0168092

Effect of Butyrate on Collagen Expression, Cell Viability, Cell Cycle Progression and Related Proteins Expression of MG-63 Osteoblastic Cells.

Chang MC, Tsai YL, Liou EJ, Tang CM, Wang TM, Liu HC, Liao MW, Yeung SY, Chan CP, Jeng JH
PloS one 2016;11(11):e0165438

K20E, an oxidative-coupling compound of methyl caffeate, exhibits anti-angiogenic activities through down-regulations of VEGF and VEGF receptor-2.

Pan CH, Lin WH, Chien YC, Liu FC, Sheu MJ, Kuo YH, Wu CH
Toxicology and applied pharmacology 2015 Jan 15;282(2):215-26

Combination therapy targeting ectopic ATP synthase and 26S proteasome induces ER stress in breast cancer cells.

Chang HY, Huang TC, Chen NN, Huang HC, Juan HF
Cell death & disease 2014 Nov 27;5:e1540

Involvement of p38 mitogen-activated protein kinase in acquired gemcitabine-resistant human urothelial carcinoma sublines

Kao Y, Hsu W, Hu H, Hsu S, Lin C, Chiu C, Lu C, Hour T, Pu Y, Huang A
The Kaohsiung Journal of Medical Sciences 2014 July;30(7):323-330

Elucidating the inhibitory mechanisms of the ethanolic extract of the fruiting body of the mushroom Antrodia cinnamomea on the proliferation and migration of murine leukemia WEHI-3 cells and their tumorigenicity in a BALB/c allograft tumor model.

Liu FC, Lai MT, Chen YY, Lin WH, Chang SJ, Sheu MJ, Wu CH
Phytomedicine : international journal of phytotherapy and phytopharmacology 2013 Jul 15;20(10):874-82

Two Cases of Metastatic Parathyroid Carcinoma in Male C3H Mice Following Irradiation.

Ohmachi Y, Yoshida M, Ogiu T
Journal of toxicologic pathology 2013 Dec;26(4):413-7

Down-regulation of type I Runx2 mediated by dexamethasone is required for 3T3-L1 adipogenesis.

Zhang YY, Li X, Qian SW, Guo L, Huang HY, He Q, Liu Y, Ma CG, Tang QQ
Molecular endocrinology (Baltimore, Md.) 2012 May;26(5):798-808

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (50 ?g of whole cell lysate) A: MFB (mouse fibroblast) 12% SDS PAGE GTX100446 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample(30 ?g of whole cell lysate)A:A431(GTX27909)12% SDS PAGEGTX100446 diluted at 1:200
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ug of whole cell lysate) A:NIH-3T312% SDS PAGE GTX100446 diluted at 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (50 ?g of whole cell lysate) A: CCRF-SB 12% SDS PAGE GTX100446 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: p27 Kip1 antibody detects p27 Kip1 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 12% SDS-PAGE, and blotted with p27 Kip1 antibody (GTX100446) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: p27 Kip1 antibody detects p27 Kip1 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody (GTX100446) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody (GTX100446) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Wild-type (WT) and p27 Kip1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody (GTX100446) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: p27 Kip1 antibody detects p27 Kip1 protein by Western blot analysis. Various whole cell extracts (30 £gg) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody (GTX100446) diluted at 1:1000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody (GTX100446) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: p27 Kip1 antibody detects p27 Kip1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: p27 Kip1 protein stained by p27 Kip1 antibody (GTX100446) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunoprecipitation of p27 Kip1 protein from MCF-7 whole cell extracts using 5 £gg of p27 Kip1 antibody (GTX100446).Western blot analysis was performed using p27 Kip1 antibody (GTX100446).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical staining of human ulcerative colitis tissue using anti-p27 Kip1 antibody (GTX100446).

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: p27 Kip1 antibody detects p27 Kip1 protein at nucleus by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: p27 Kip1 protein stained by p27 Kip1 antibody (GTX100446) diluted at 1:250.Red: Protein kinase C alpha staining.Blue: Fluoroshield with DAPI (GTX30920).

GTX100446

Antibody data