- AHZ0452 - Invitrogen Antibodies CDKN1B antibody

Submitted references Response of neuroblastoma cells to RF currents as a function of the signal frequency.

Hernández-Bule ML, Medel E, Colastra C, Roldán R, Úbeda A
BMC cancer 2019 Sep 5;19(1):889

Evaluating the expression of p16 and p27 in oral epithelial dysplasias and oral squamous cell carcinoma: A diagnostic marker for carcinogenesis.

Thambiah LJ, Bindushree RV, Anjum A, Pugazhendi SK, Babu L, Nair RP
Journal of oral and maxillofacial pathology : JOMFP 2018 Jan-Apr;22(1):59-64

Induction of Inner Ear Hair Cells from Mouse Embryonic Stem Cells In Vitro.

Yoshikawa M, Ouji Y
Methods in molecular biology (Clifton, N.J.) 2016;1516:257-267

Power Frequency Magnetic Fields Affect the p38 MAPK-Mediated Regulation of NB69 Cell Proliferation Implication of Free Radicals.

Martínez MA, Úbeda A, Moreno J, Trillo MÁ
International journal of molecular sciences 2016 Apr 6;17(4):510

A kinome-targeted RNAi-based screen links FGF signaling to H2AX phosphorylation in response to radiation.

Benzina S, Pitaval A, Lemercier C, Lustremant C, Frouin V, Wu N, Papine A, Soussaline F, Romeo PH, Gidrol X
Cellular and molecular life sciences : CMLS 2015 Sep;72(18):3559-73

The RNA-binding protein LIN28B regulates developmental timing in the mammalian cochlea.

Golden EJ, Benito-Gonzalez A, Doetzlhofer A
Proceedings of the National Academy of Sciences of the United States of America 2015 Jul 21;112(29):E3864-73

Synergistic Effect of Hyperglycemia and p27(kip1) Suppression on Adult Mouse Islet Beta Cell Replication.

Chen ST, Fu SH, Hsu S, Huang YY, Hsu BR
International journal of endocrinology 2012;2012:417390

Injury-independent induction of reactive gliosis in retina by loss of function of the LIM homeodomain transcription factor Lhx2.

de Melo J, Miki K, Rattner A, Smallwood P, Zibetti C, Hirokawa K, Monuki ES, Campochiaro PA, Blackshaw S
Proceedings of the National Academy of Sciences of the United States of America 2012 Mar 20;109(12):4657-62

Identification of prognostic biomarkers for glioblastomas using protein expression profiling.

Jung Y, Joo KM, Seong DH, Choi YL, Kong DS, Kim Y, Kim MH, Jin J, Suh YL, Seol HJ, Shin CS, Lee JI, Kim JH, Song SY, Nam DH
International journal of oncology 2012 Apr;40(4):1122-32

Shp2 acts downstream of SDF-1alpha/CXCR4 in guiding granule cell migration during cerebellar development.

Hagihara K, Zhang EE, Ke YH, Liu G, Liu JJ, Rao Y, Feng GS
Developmental biology 2009 Oct 1;334(1):276-84

Differential modification of p27Kip1 controls its cyclin D-cdk4 inhibitory activity.

James MK, Ray A, Leznova D, Blain SW
Molecular and cellular biology 2008 Jan;28(1):498-510

PTEN expression in PTEN-null leukaemic T cell lines leads to reduced proliferation via slowed cell cycle progression.

Seminario MC, Precht P, Wersto RP, Gorospe M, Wange RL
Oncogene 2003 Nov 6;22(50):8195-204

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of P27Kip1/CDKN1B in LS174T cells using a P27Kip1/CDKN1B monoclonal antibody (Product # AHZ0452).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of p27Kip1 in Hela cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a p27Kip1 monoclonal antibody (Product # AHZ0452) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of p27Kip1 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a p27Kip1 monoclonal antibody (Product # AHZ0452) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of p27Kip1 in NIH-3T3 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a p27Kip1 monoclonal antibody (Product # AHZ0452) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Field effects on cell cycle regulatory proteins. ( A ) Expression of cyclin D1 and p27 kip1 after 120 min of MF- or sham-exposure, using beta-actin as loading control. The data, normalized over the respective values of protein expression in sham-exposed controls (line 100%), are means +- SEM of six experimental replicates. Statistically significant differences with respect to controls: 0.01

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of P27Kip1/CDKN1B in native mouse MAD109 cells using a P27Kip1/CDKN1B monoclonal antibody (Product # AHZ0452).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 3 Validation of the screening results by Western blotting. a Eight kinases selected in the screen were randomly chosen for further validation. Seven of them exhibited reduced expression at the protein level upon siRNA-mediated knockdown and reduced phosphorylation of H2AX in HaCaT cells after irradiation at 2 Gy. b Knockdown of JNK1 and FGFR4 reduced the phosphorylation of H2AX in primary keratinocytes after irradiation at 2 Gy. c Clonogenic assay on HaCaT cells irradiated at 2, 4, and 6 Gy and previously transfected with siCTL, siATM, siJNK1, siFGFR4 or a combination of the last three siRNAs (siCOCK for cocktail). Error bars represent standard deviation

AHZ0452

Antibody data