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- Product number
- 600-401-K08S - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-K08S, RRID:AB_2614460
- Product name
- Anti-p27 kip1 pS140 (RABBIT) Antibody - 600-401-K08S
- Antibody type
- Polyclonal
- Vial size
- 25 µl
Submitted references Upregulation of human glycolipid transfer protein (GLTP) induces necroptosis in colon carcinoma cells.
Mishra SK, Stephenson DJ, Chalfant CE, Brown RE
Biochimica et biophysica acta. Molecular and cell biology of lipids 2019 Feb;1864(2):158-167
Biochimica et biophysica acta. Molecular and cell biology of lipids 2019 Feb;1864(2):158-167
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
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- Experimental details
- Western Blot of Rabbit anti-p27kip1pS140 antibody. Lane 1: 293 cells untransfected. Lane 2: 293 cells untransfected and 5Gy irradiated. Lane 3: 293 cells transfected with HA-p27wt. Lane 4: 293 cells transfected with HA-p27wt and 5Gy irradiated. Lane 5: 293 cells transfected with HA-p27S140A. Lane 6: 293 cells transfected with HA-p27S140A and 5Gy irradiated. Load: 20 µg per lane. Primary antibody: p27kip1pS140 antibody at 1:250 for overnight at 4°C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TTBS overnight at 4°C. Predicted/Observed size: 27 kDa for p27kip1.
- Validation comment
- Western Blot
