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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 602901 - Provider product page
- Provider
- BioLegend
- Product name
- Purified anti-p27Kip1
- Antibody type
- Polyclonal
- Antigen
- Peptide derived from the carboxy terminus of human p27
- Description
- p27Kip1 (cyclin-dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor p27) is a 27kD cyclin-dependent inhibitor. p27Kip1 is localized in the nucleus and cytoplasm (phospho-specific). This protein functions in G1 arrest and binds and inhibits complexes formed by cyclin E-CDK2, cyclin A-CDK2. p27Kip1 mediates TGF β-induced G1 arrest and regulates cell motility. This kinase has also been implicated in translational control. Akt phosphorylation causes p27 Kip1 export to cytoplasm; p27 Kip1 has also been shown to be ubiquitinated. p27 Kip1 has been shown to interact with NUP50, Skp2, and PKB/Akt. The Poly6029 antibody recognizes the C-terminal region of human p27Kip1 and has been shown to be useful for Western blotting.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 50 µl
- Storage
- The antibody solution should be stored undiluted at 4° C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-p27Kip1 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
- Conjugate
- Unconjugated
