MA5-12835

antibody from Invitrogen Antibodies

Targeting: CDKN1B KIP1, P27KIP1

Immunocytochemistry

Antibody data

Submit

Validation data

Product number: MA5-12835 - Provider product page
Provider: Invitrogen Antibodies
Product name: Anti-p27Kip1 Monoclonal Antibody (DCS-72.F6)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: MA5-12835 targets p27Kip1 in FACS, IF, IP, and WB applications and shows reactivity with Canine, Human, mouse, and Rat samples. The MA5-12835 immunogen is mouse recombinant p27 protein.
Reactivity: Human, Mouse, Rat, Canine
Host: Mouse
Isotype: IgG
Antibody clone number: DCS-72.F6
Vial size: 500 µL
Concentration: 0.2 mg/mL
Storage: 4° C

CDKN1B protein structure - MA5-12835 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of p27Kip1 in Hela cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a p27Kip1 monoclonal antibody (Product # MA5-12835) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of p27Kip1 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a p27Kip1 monoclonal antibody (Product # MA5-12835) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of p27Kip1 in NIH-3T3 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a p27Kip1 monoclonal antibody (Product # MA5-12835) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.