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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-30544 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NARF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, H1299, HCT116, MCF-7, mouse muscle.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NARF Polyclonal Antibody (Product # PA5-30544). Sample (50 µg of whole cell lysate). Lane A: mouse muscle . 7.5% SDS PAGE. NARF Polyclonal Antibody (Product # PA5-30544) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NARF Polyclonal Antibody (Product # PA5-30544). Sample (30 µg of whole cell lysate). Lane A: A549. Lane B: H1299. Lane C: HCT116. Lane D: MCF-7 . 7.5% SDS PAGE. NARF Polyclonal Antibody (Product # PA5-30544) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NARF Polyclonal Antibody detects NARF protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NARF protein stained by NARF Polyclonal Antibody (Product # PA5-30544) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using NARF (Product # PA5-30544) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
