PA5-27586

antibody from Invitrogen Antibodies

Targeting: APLP2 APPH, APPL2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27586

Provider

Invitrogen Antibodies

Product name

APLP2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain.

Concentration

0.23 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of APLP2 using A) 30 µg 293T whole cell lysate (B) 30 µg A431 whole cell lysate (C) 30 µg HeLa whole cell lysate (D) 30 µg HepG2 whole cell lysate and E) 30 µg A375 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an APLP2 polyclonal antibody (Product # PA5-27586) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of APLP2 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a APLP2 Polyclonal Antibody (Product # PA5-27586) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of APLP2 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a APLP2 Polyclonal Antibody (Product # PA5-27586) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

APLP2 Polyclonal Antibody detects APLP2 protein by Western blot analysis. A. 50 µg mouse brain lysate/extract.7.5 % SDS-PAGE. APLP2 Polyclonal Antibody (Product # PA5-27586) dilution: 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

APLP2 Polyclonal Antibody detects APLP2 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. APLP2 Polyclonal Antibody (Product # PA5-27586) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

APLP2 Polyclonal Antibody detects APLP2 protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. APLP2 Polyclonal Antibody (Product # PA5-27586) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.