PA5-47031

antibody from Invitrogen Antibodies

Targeting: ADAMTS8 ADAM-TS8, FLJ41712, METH2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47031

Provider

Invitrogen Antibodies

Product name

ADAMTS8 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) ADAMTS1 is observed and less than 1% cross-reactivity with rhADAMTS1, rhADAMTS3, rhADAMTS4, rhADAMTS5, rhADAMTS10, rhADAMTS12, rhADAMTS13, and rhADAMTS16 is observed.

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of 293T human embryonic kidney cell line either transfected with human ADAMTS8, mock transfected (-), or transfected with an unrelated vector. PVDF Membrane was probed with 1 µg/mL of human ADAMTS8 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47031) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for ADAMTS8 at approximately 80-85 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ADAMTS8 in 293T human embryonic kidney cell line either transfected with human ADAMTS8, mock transfected (-), or transfected with an unrelated vector. Samples were incubated in ADAMTS8 polyclonal antibody (Product # PA5-47031) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for ADAMTS8 at approximately 80-85 kDa (as indicated). This experiment was conducted under reducing conditions.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of ADAMTS8 in immersion fixed paraffin-embedded sections of human brain (cerebellum). Samples were incubated in ADAMTS8 polyclonal antibody (Product # PA5-47031) using a dilution of 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic . Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons.