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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-28182 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLC20A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299, mouse brain.
- Concentration
- 0.89 mg/mL
Submitted references Magnesium but not nicotinamide prevents vascular calcification in experimental uraemia.
Kaesler N, Goettsch C, Weis D, Schurgers L, Hellmann B, Floege J, Kramann R
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2020 Jan 1;35(1):65-73
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2020 Jan 1;35(1):65-73
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SLC20A1 using 50 µg of mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a SLC20A1 polyclonal antibody (Product # PA5-28182) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SLC20A1 using 30 µg of H1299 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a SLC20A1 polyclonal antibody (Product # PA5-28182) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SLC20A1 was performed by separating 30 µg of Unboiled U87-MG cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a SLC20A1 Polyclonal Antibody (Product # PA5-28182) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SLC20A1 Polyclonal Antibody detects SLC20A1 protein at cytosol and membrane on AGS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded AGS xenograft. SLC20A1 Polyclonal Antibody (Product # PA5-28182) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SLC20A1 Polyclonal Antibody detects SLC20A1 protein at membrane and cytoplasm on mouse mammary gland by immunohistochemical analysis. Sample: Paraffin-embedded mouse mammary gland. SLC20A1 Polyclonal Antibody (Product # PA5-28182) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
