Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [10]
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Validation data
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- Product number
- NBP1-85886 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-85886, RRID:AB_11036060
- Product name
- Rabbit Polyclonal PCCB Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human PCCB antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: PCCB Antibody [NBP1-85886] - Simple Western lane view shows a specific band for PCCB in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: PCCB Antibody [NBP1-85886] - Electropherogram image(s) of corresponding Simple Western lane view. PCCB antibody was used at 1:20 dilution on RT-4 and U-251MG sp lysates(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCCB Antibody [NBP1-85886] - Analysis in human cell line HepG2.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCCB Antibody [NBP1-85886] - Analysis using Anti-PCCB antibody NBP1-85886 (A) shows similar pattern to independent antibody NBP1-85887 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886] - Staining of human rectum shows strong granular cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886] - Staining of human skin shows only weak cytoplasmic positivity in a subset of epidermal cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886] - Staining in human liver and skin tissues . Corresponding PCCB RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886] - Staining of human kidney, liver, salivary gland and tonsil using Anti-PCCB antibody NBP1-85886 (A) shows similar protein distribution across tissues to independent antibody NBP1-85887 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886] - Staining of human salivary gland.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886] - Staining of human liver using Anti-PCCB antibody NBP1-85886.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886] - Staining of human tonsil.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886] - Staining of human kidney using Anti-PCCB antibody NBP1-85886.