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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [3]
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- Product number
- MA5-24785 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLR2D Monoclonal Antibody (RB42)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Human plasma RBP4. All MAbs recognize free RBP4 and RBP4 in complex with transthyretin. All MAbs react with apo- as well as holo-RBP4.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- RB42
- Vial size
- 200 µg
- Storage
- 4° C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of POLR2D in RB42, RB45, RB48 and RB51. Samples were incubated in POLR2D monoclonal antibody (Product # MA5-24785). Lane 1: MAb RB42; Lane 2: RB45; Lane 3: RB48; Lane 4: RB51. Reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA analysis of RBP4/Retinol-Binding Protein 4/POLR2D monoclonal antibody (Product # MA5-24785) was performed using a dilution of 1.0 µg/mL per well. Detection antibody was labeled a stable Eu3+ chelate using a dilution of 0.2 µg/well.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA of POLR2D in purified endogenous RBP4. Samples were incubated in POLR2D monoclonal antibody (Product # MA5-24785). In the presence of 5 mM EDTA (blue line) or in absence of EDTA (pink line). Capture antibody: RB48 (1 µg/well). Detection antibody: RB42 labeled with stable Eu3+ chelate (0.2 µg/well). Antigen: Native isolated RBP4.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA of POLR2D in native free and native complexed RBP4. Samples were incubated in POLR2D monoclonal antibody (Product # MA5-24785). Sample after several freeze-thaw cycles measured with the assay RB48 - RB42. Capture antibody: RB48 (1 µg/well). Detection antibody: RB42 labeled with stable Eu3+ chelate (0.2 µg/well). Antigen: Native isolated RBP4.
