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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [14]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF462 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse TRANCE/TNFSF11/RANK L Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse TRANCE/TNFSF11/RANK L in ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse (rm) CD27, rmCD40, and rmFas is observed and less than 1% cross-reactivity with rmCD30, recombinant human (rh) DR6, rmGITR, rmTNF RI, and rmTNF RII is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O35235- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references RIPK1 inhibition attenuates experimental autoimmune arthritis via suppression of osteoclastogenesis.
Soluble RANKL contributes to osteoclast formation in adult mice but not ovariectomy-induced bone loss.
Attenuation of RNA polymerase II pausing mitigates BRCA1-associated R-loop accumulation and tumorigenesis.
The ω-3 polyunsaturated fatty acid, eicosapentaenoic acid, attenuates abdominal aortic aneurysm development via suppression of tissue remodeling.
Autophagy regulator BECN1 suppresses mammary tumorigenesis driven by WNT1 activation and following parity.
Paracrine-rescued lobulogenesis in chimeric outgrowths comprising progesterone-receptor-null mammary epithelium and redirected wild-type testicular cells.
Fibromodulin and Biglycan Modulate Periodontium through TGFβ/BMP Signaling.
CXCL5 stimulation of RANK ligand expression in Paget's disease of bone.
Osteoclast differentiation factor RANKL controls development of progestin-driven mammary cancer.
The RANKL signaling axis is sufficient to elicit ductal side-branching and alveologenesis in the mammary gland of the virgin mouse.
Estrogen and progesterone are critical regulators of Stat5a expression in the mouse mammary gland.
Osteoprotegerin inhibits vascular calcification without affecting atherosclerosis in ldlr(-/-) mice.
IL-17 promotes bone erosion in murine collagen-induced arthritis through loss of the receptor activator of NF-kappa B ligand/osteoprotegerin balance.
IL-17 promotes bone erosion in murine collagen-induced arthritis through loss of the receptor activator of NF-kappa B ligand/osteoprotegerin balance.
Jhun J, Lee SH, Kim SY, Ryu J, Kwon JY, Na HS, Jung K, Moon SJ, Cho ML, Min JK
Journal of translational medicine 2019 Mar 15;17(1):84
Journal of translational medicine 2019 Mar 15;17(1):84
Soluble RANKL contributes to osteoclast formation in adult mice but not ovariectomy-induced bone loss.
Xiong J, Cawley K, Piemontese M, Fujiwara Y, Zhao H, Goellner JJ, O'Brien CA
Nature communications 2018 Jul 25;9(1):2909
Nature communications 2018 Jul 25;9(1):2909
Attenuation of RNA polymerase II pausing mitigates BRCA1-associated R-loop accumulation and tumorigenesis.
Zhang X, Chiang HC, Wang Y, Zhang C, Smith S, Zhao X, Nair SJ, Michalek J, Jatoi I, Lautner M, Oliver B, Wang H, Petit A, Soler T, Brunet J, Mateo F, Angel Pujana M, Poggi E, Chaldekas K, Isaacs C, Peshkin BN, Ochoa O, Chedin F, Theoharis C, Sun LZ, Curiel TJ, Elledge R, Jin VX, Hu Y, Li R
Nature communications 2017 Jun 26;8:15908
Nature communications 2017 Jun 26;8:15908
The ω-3 polyunsaturated fatty acid, eicosapentaenoic acid, attenuates abdominal aortic aneurysm development via suppression of tissue remodeling.
Wang JH, Eguchi K, Matsumoto S, Fujiu K, Komuro I, Nagai R, Manabe I
PloS one 2014;9(5):e96286
PloS one 2014;9(5):e96286
Autophagy regulator BECN1 suppresses mammary tumorigenesis driven by WNT1 activation and following parity.
Cicchini M, Chakrabarti R, Kongara S, Price S, Nahar R, Lozy F, Zhong H, Vazquez A, Kang Y, Karantza V
Autophagy 2014;10(11):2036-52
Autophagy 2014;10(11):2036-52
Paracrine-rescued lobulogenesis in chimeric outgrowths comprising progesterone-receptor-null mammary epithelium and redirected wild-type testicular cells.
Bruno RD, Boulanger CA, Rosenfield SM, Anderson LH, Lydon JP, Smith GH
Journal of cell science 2014 Jan 1;127(Pt 1):27-32
Journal of cell science 2014 Jan 1;127(Pt 1):27-32
Fibromodulin and Biglycan Modulate Periodontium through TGFβ/BMP Signaling.
Wang L, Foster BL, Kram V, Nociti FH Jr, Zerfas PM, Tran AB, Young MF, Somerman MJ
Journal of dental research 2014 Aug;93(8):780-7
Journal of dental research 2014 Aug;93(8):780-7
CXCL5 stimulation of RANK ligand expression in Paget's disease of bone.
Sundaram K, Rao DS, Ries WL, Reddy SV
Laboratory investigation; a journal of technical methods and pathology 2013 Apr;93(4):472-9
Laboratory investigation; a journal of technical methods and pathology 2013 Apr;93(4):472-9
Osteoclast differentiation factor RANKL controls development of progestin-driven mammary cancer.
Schramek D, Leibbrandt A, Sigl V, Kenner L, Pospisilik JA, Lee HJ, Hanada R, Joshi PA, Aliprantis A, Glimcher L, Pasparakis M, Khokha R, Ormandy CJ, Widschwendter M, Schett G, Penninger JM
Nature 2010 Nov 4;468(7320):98-102
Nature 2010 Nov 4;468(7320):98-102
The RANKL signaling axis is sufficient to elicit ductal side-branching and alveologenesis in the mammary gland of the virgin mouse.
Fernandez-Valdivia R, Mukherjee A, Ying Y, Li J, Paquet M, DeMayo FJ, Lydon JP
Developmental biology 2009 Apr 1;328(1):127-39
Developmental biology 2009 Apr 1;328(1):127-39
Estrogen and progesterone are critical regulators of Stat5a expression in the mouse mammary gland.
Santos SJ, Haslam SZ, Conrad SE
Endocrinology 2008 Jan;149(1):329-38
Endocrinology 2008 Jan;149(1):329-38
Osteoprotegerin inhibits vascular calcification without affecting atherosclerosis in ldlr(-/-) mice.
Morony S, Tintut Y, Zhang Z, Cattley RC, Van G, Dwyer D, Stolina M, Kostenuik PJ, Demer LL
Circulation 2008 Jan 22;117(3):411-20
Circulation 2008 Jan 22;117(3):411-20
IL-17 promotes bone erosion in murine collagen-induced arthritis through loss of the receptor activator of NF-kappa B ligand/osteoprotegerin balance.
Lubberts E, van den Bersselaar L, Oppers-Walgreen B, Schwarzenberger P, Coenen-de Roo CJ, Kolls JK, Joosten LA, van den Berg WB
Journal of immunology (Baltimore, Md. : 1950) 2003 Mar 1;170(5):2655-62
Journal of immunology (Baltimore, Md. : 1950) 2003 Mar 1;170(5):2655-62
IL-17 promotes bone erosion in murine collagen-induced arthritis through loss of the receptor activator of NF-kappa B ligand/osteoprotegerin balance.
Lubberts E, van den Bersselaar L, Oppers-Walgreen B, Schwarzenberger P, Coenen-de Roo CJ, Kolls JK, Joosten LA, van den Berg WB
Journal of immunology (Baltimore, Md. : 1950) 2003 Mar 1;170(5):2655-62
Journal of immunology (Baltimore, Md. : 1950) 2003 Mar 1;170(5):2655-62
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Osteoclast-like Cell Formation Induced by TRANCE/ TNFSF11/RANK L and Neutralization by Mouse TRANCE/TNFSF11/RANK L Antibody. In the presence of Recombinant Mouse M-CSF (20 ng/mL, Catalog # 416-ML), Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TR) induces osteoclast-like cell formation in mouse splenocytes in a dose-dependent manner (orange line), as measured by TRAP (tartrate-resistant acid phosphatase) solution assay. Under these conditions, osteoclast-like cell formation elicited by Recombinant Mouse TRANCE/TNFSF11/ RANK L (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TRANCE/TNFSF11/RANK L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF462). The ND50 is typically 0.02-0.06 µg/mL.
