Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 200-301-E95 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-301-E95, RRID:AB_2611124
- Product name
- Anti-BK channel BETA2 (MOUSE) Monoclonal Antibody
- Antibody type
- Monoclonal
- Antigen
- Recombinant Protein
- Description
- Anti-BK channel BETA2 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with BK channel BETA2 from human, mouse, and rat based on 100% homology with the immunizing sequence. Detects endogenous and exogenous hsp22 in monomeric, dimeric and tetrameric forms in WB. Does not cross react with alpha crystallin. Cross-reactivity with BK channel BETA2 from other sources has not been determined. Ion Channels research.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 1mg/mL by UV absorbance at 280 nm
- Storage
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Handling
- Dry Ice
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Mouse anti-BK channel BETA2 antibody. Lane 1: COS-1 cells transiently transfected with KBeta2 lysate. Load: 10ug. Priamary antibody: BK channel BETA2 at 1:1000 overnight at 4°C. Secondary antibody: Goat anti-mouse IgG HRP at 1:40,000 for 45 min at RT. Blocked: 5% Blotto overnight at 4°C. Predicated/observed size: 27.1 kDa, 30 kDa for BK channel BETA2.
- Validation comment
- Western Blot