PA5-78606

antibody from Invitrogen Antibodies

Targeting: PSRC1 DDA3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-78606

Provider

Invitrogen Antibodies

Product name

PSRC1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: 293T, A431, human PSRC1-transfected 293T

Concentration

0.86 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PSRC1 in whole cell lysate using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with PSRC1 polyclonal antibody (Product # PA5-78606) using a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PSRC1 in A) 293T whole cell lysate, B) whole cell lysate of human PSRC1-transfected 293T cells using 30 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with PSRC1 polyclonal antibody (Product # PA5-78606) using a dilution of 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PSRC1 Polyclonal Antibody detects PSRC1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSRC1 Polyclonal Antibody (Product # PA5-78606) diluted by 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PSRC1 Polyclonal Antibody detects PSRC1 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of human PSRC1-transfected 293T cells.12 % SDS-PAGE. PSRC1 Polyclonal Antibody (Product # PA5-78606) dilution: 1:10,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PSRC1 Polyclonal Antibody detects PSRC1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PSRC1 protein stained by PSRC1 Polyclonal Antibody (Product # PA5-78606) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of PSRC1 was performed in paraffin-embedded mouse brain tissue using PSRC1 Polyclonal Antibody (Product # PA5-78606) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Frozen) analysis of PSRC1 was performed in frozen-sectioned mouse mouse cerebellum tissue using PSRC1 Polyclonal Antibody (Product # PA5-78606) at a dilution of 1:250 (Green). Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI.