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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- ARG54646 - Provider product page
- Provider
- Arigo
- Product name
- anti-ATP11B antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide around 19 aa (n-terminus) of Human ATP11B protein (NP_055431.1).
- Description
- Immunogen affinity purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 1 mg/ml
- Storage
- For continuous use, store undiluted antibody at 4°C for up to 3 months. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 1) K562 cell tissue lysate without blocking peptide 2) K562 cell tissue lysate with blocking peptide stained with ARG54646 anti-ATP11B antibody. Lysates/proteins at 15 µg per lane.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunocytochemistry: ATP11B in K562 cells stained with ARG54646 anti-ATP11B antibody at 10 µg/ml.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: ATP11B in K562 cells stained with ARG54646 anti-ATP11B antibody at 20 µg/ml.
