H00009601-M01

antibody from Abnova Corporation

Targeting: PDIA4 ERP70, ERP72

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00009601-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00009601-M01, RRID:AB_464341

Product name

PDIA4 monoclonal antibody (M01), clone 4C6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant PDIA4.

Antigen sequence

VAGAEGPDEDSSNRENAIEDEEEEEEEDDDEEEDD
LEVKEENGVLVLNDANFDNFVADKDTVLLEFYAPW
CGHCKQFAPEYEKIANILKDKDPPIPVAKIDATSA
SVLASRFDVSGYPTIKILKKGQAVDYEGSRTQEEI
VAKVREVSQPDWTPPPEVTLVLTKENFDEVVNDAD
IILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPI
PLAKVDATAETDLAKRFDVSGYPTLKIFRKGRPYD
YNGPREKYGIVDYMIEQSGPPSKEILTLKQVQEFL
KDGDDVIIIGVFKGESDPAYQQYQDAANNLREDYK
FHHTFSTEIAKFLKVSQGQLVVMQPEKFQSKYEPR
SHMMDVQGSTQDSAIKDFVLKYALPLVGHRKVSND
AKRYTRRPLVVVYYSVDFSFDYRAATQFWRSKVLE
VAKDFPEYTFAIADEEDYAGEVKDLGLSESGEDVN
AAILDESGKKFAMEPEEFDSDTLREFVTAFKKGKL
KPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKD
VLIEFYAPWCGHCKQLEPVYNSLAKKYKGQKGLVI
AKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPV
KFEGGDRDLEHLSKFIEEHATKLSRTKEEL


Isotype

IgG

Antibody clone number

4C6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PDIA4 expression in transfected 293T cell line by PDIA4 monoclonal antibody (M01), clone 4C6.Lane 1: PDIA4 transfected lysate (Predicted MW: 72.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PDIA4 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol