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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- H00009601-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00009601-M02, RRID:AB_1137354
- Product name
- PDIA4 monoclonal antibody (M02), clone 2H3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant PDIA4.
- Antigen sequence
LVVMQPEKFQSKYEPRSHMMDVQGSTQDSAIKDFV
LKYALPLVGHRKVSNDAKRYTRRPLVVVYYSVDFS
FDYRAATQFWRSKVLEVAKDFPEYTFAIADEEDYA
GEVKD- Isotype
- IgG
- Antibody clone number
- 2H3
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PDIA4 expression in transfected 293T cell line by PDIA4 monoclonal antibody (M02), clone 2H3.Lane 1: PDIA4 transfected lysate (Predicted MW: 72.9 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PDIA4 is approximately 3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
