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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA503904 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA503904, RRID:AB_11124360
- Product name
- PDIA4 (ERp72) mouse monoclonal antibody, clone OTI2E2 (formerly 2E2)
- Antibody type
- Monoclonal
- Description
- PDIA4 (ERp72) mouse monoclonal antibody, clone OTI2E2 (formerly 2E2)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PDIA4
- Isotype
- IgG
- Antibody clone number
- OTI2E2
- Vial size
- 100 µl
- Concentration
- 0.87 mg/ml
Submitted references Metformin alleviates human cellular aging by upregulating the endoplasmic reticulum glutathione peroxidase 7.
Fang J, Yang J, Wu X, Zhang G, Li T, Wang X, Zhang H, Wang CC, Liu GH, Wang L
Aging cell 2018 Aug;17(4):e12765
Aging cell 2018 Aug;17(4):e12765
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDIA4 (RC204041, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDIA4.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDIA4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-PDIA4 mouse monoclonal antibody (TA503904) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDIA4(RC204041).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC204041 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDIA4 antibody(TA503904), and then analyzed by flow cytometry.
- Validation comment
- FC
