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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- ELISA [1]
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- Product number
- H00010286-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00010286-M01, RRID:AB_2274635
- Product name
- Mouse Monoclonal BCAS2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. BCAS2 - breast carcinoma amplified sequence 2
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references A quantitative proteomic analysis uncovers the relevance of CUL3 in bladder cancer aggressiveness.
Grau L, Luque-Garcia JL, González-Peramato P, Theodorescu D, Palou J, Fernandez-Gomez JM, Sánchez-Carbayo M
PloS one 2013;8(1):e53328
PloS one 2013;8(1):e53328
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BCAS2 Antibody (1D10) [H00010286-M01] - Analysis of BCAS2 over-expressed 293 cell line, cotransfected with BCAS2 Validated Chimera RNAi ( Cat # H00010286-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with BCAS2 monoclonal antibody (M01), clone 1D10 (Cat # H00010286-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BCAS2 Antibody (1D10) [H00010286-M01] - Analysis of BCAS2 expression in transfected 293T cell line by BCAS2 monoclonal antibody (M01), clone 1D10.Lane 1: BCAS2 transfected lysate(26.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BCAS2 Antibody (1D10) [H00010286-M01] - BCAS2 monoclonal antibody (M01), clone 1D10 Analysis of BCAS2 expression in K-562.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: BCAS2 Antibody (1D10) [H00010286-M01] - Detection limit for recombinant GST tagged BCAS2 is approximately 1ng/ml as a capture antibody.
