PA5-30373

antibody from Invitrogen Antibodies

Targeting: BCAS2 DAM1, Snt309, SPF27

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30373

Provider

Invitrogen Antibodies

Product name

BCAS2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat-2, NT2D1, IMR32, MCF-7.

Concentration

0.43 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of BCAS2 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a BCAS2 Polyclonal Antibody (Product # PA5-30373) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of BCAS2 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a BCAS2 Polyclonal Antibody (Product # PA5-30373) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using BCAS2 Polyclonal Antibody (Product # PA5-30373). Sample (30 µg of whole cell lysate). Lane A: MCF-7. 12% SDS PAGE. BCAS2 Polyclonal Antibody (Product # PA5-30373) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of BCAS2 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a BCAS2 Polyclonal Antibody (Product # PA5-30373) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of BCAS2 in paraformaldehyde-fixed HeLa cells using a BCAS2 polyclonal antibody (Product # PA5-30373) at a 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded J5 xenograft, using BCAS2 (Product # PA5-30373) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.