Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB110-40681 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-40681, RRID:AB_2063401
- Product name
- Rabbit Polyclonal BCAS2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references DDB2 is a novel AR interacting protein and mediates AR ubiquitination/degradation.
The RNA polymerase II C-terminal domain promotes splicing activation through recruitment of a U2AF65-Prp19 complex.
Chang SW, Su CH, Chen HH, Huang CW, Tsao LP, Tsao YP, Chen SL
The international journal of biochemistry & cell biology 2012 Nov;44(11):1952-61
The international journal of biochemistry & cell biology 2012 Nov;44(11):1952-61
The RNA polymerase II C-terminal domain promotes splicing activation through recruitment of a U2AF65-Prp19 complex.
David CJ, Boyne AR, Millhouse SR, Manley JL
Genes & development 2011 May 1;25(9):972-83
Genes & development 2011 May 1;25(9):972-83
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BCAS2 Antibody [NB110-40681] - Detection of Human and Mouse BCAS2 by Western Blot. Samples: Whole cell lysate from MCF7 (5, 15 and 50 ug), and HeLa (50ug), and mouse NIH3T3 (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BCAS2 antibody NB110-40681 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: BCAS2 Antibody [NB110-40681] - Detection of Human BCAS2 on HeLa whole cell lysates using NB110-40681. BCAS2 was also immunoprecipitated by other rabbit anti-BCAS2 antibodies.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: BCAS2 Antibody [NB110-40681] - Detection of human BCAS2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BCAS2 antibody NB110-40681 (lot NB110-40681-2) used for IP at 6 ug per reaction. BCAS2 was also immunoprecipitated by a previous lot of this antibody (lot NB110-40681-1) and another rabbit anti-BCAS2 antibody. For blotting immunoprecipitated BCAS2, NB110-40681 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BCAS2 Antibody [NB110-40681] - Mouse plasmacytoma. Antibody: Affinity purified rabbit anti-BCAS2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BCAS2 Antibody [NB110-40681] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BCAS2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.