Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C209203 - Provider product page
- Provider
- LSBio
- Product name
- AKT3 Antibody (Internal, clone 9A5.H9.G7) LS-C209203
- Antibody type
- Monoclonal
- Description
- Protein A affinity chromatography
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 9A5.H9.G7
- Storage
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of Mouse monoclonal anti-AKT3 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Load: 50 ng per lane. Primary antibody: anti-AKT3 antibody at 1:1000 for overnight at 4 degrees C. Secondary antibody: Rabbit secondary antibody anti mouse at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4 degrees C. Predicted/Observed size: 85 kDa for GST-AKT3. This image was taken for the unmodified form of this product. Other forms have not been tested.