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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [3]
- Flow cytometry [2]
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- Product number
- GTX54530 - Provider product page
- Provider
- GeneTex
- Product name
- GATA4 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of HeLa nuclear fraction (NF), HEK293T control and GATA4 over-expressing HEK293T lysates using GATA4 antibody at a dilution of 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of HeLa cells with (right) or without (left) GATA4 antibody at a dilution of 1:200 (green). F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a magnification of 60x.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of HepG2 cells with (right) or without (left) GATA4 antibody at a dilution of 1:200 (green). F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a magnification of 60x.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of NIH-3T3 cells with (right) or without (left) GATA4 antibody at a dilution of 1:100 (green). F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a magnification of 60x.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of mouse pancreas tissues with (right) or without (left) GATA4 antibody at a dilution of 1:500. To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of human pancreas tissues with (right) or without (left) GATA4 antibody at a dilution of 1:200. To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of human small intestine tissues with (right) or without (left) GATA4 antibody. To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FACS analysis of HepG2 cells using GATA4 antibody (green) or an isotype control (blue).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FACS analysis of HeLa cells using GATA4 antibody (green) or an isotype control (blue).
