M00021-1

antibody from Boster Biological Technology

Targeting: IL10 CSIF, IL-10, IL10A, TGIF

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

M00021-1

Provider

Boster Biological Technology

Product name

Anti- IL-10 Monoclonal Antibody

Antibody type

Monoclonal

Description

Rat IgG monoclonal antibody for IL-10 detection. Tested with WB, IHC-P in Mouse.

Reactivity

Mouse

Host

Rat

Antibody clone number

AFIE-9

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of IL-10 using anti- IL-10 antibody (M00021-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant mouse IL-10 protein 15ng, Lane 2: recombinant mouse IL-10 protein 10ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rat anti- IL-10 antigen affinity purified monoclonal antibody (Catalog # M00021-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rat IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.

Additional image