Antibody data
- Antibody Data
- Antigen structure
- References [77]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [5]
- Immunohistochemistry [3]
- Other assay [10]
Submit
Validation data
Reference
Comment
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- Product number
- MA5-13862 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Ezrin Monoclonal Antibody (3C12)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Description
- MA5-13862 targets Ezrin in IHC (P), ICC, IP, IF, and WB applications and shows reactivity with Bovine, Hamster, Human, Marsupial, mouse, Non-human primate, and Rat samples. The MA5-13862 immunogen is a recombinant fragment of human ezrin corresponding to aa 362-585.
- Reactivity
- Human, Mouse, Rat, Bovine, Hamster
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3C12
- Vial size
- 500 µL
- Concentration
- 0.2 mg/mL
- Storage
- 4° C
Submitted references Oxidative stress differentially impacts apical and basolateral secretion of angiogenic factors from human iPSC-derived retinal pigment epithelium cells.
A Structurally Conserved RNA Element within SARS-CoV-2 ORF1a RNA and S mRNA Regulates Translation in Response to Viral S Protein-Induced Signaling in Human Lung Cells.
Astrovirus-induced epithelial-mesenchymal transition via activated TGF-β increases viral replication.
Role of FGF and Hyaluronan in Choroidal Neovascularization in Sorsby Fundus Dystrophy.
Mechanism of Dyslipidemia in Obesity-Unique Regulation of Ileal Villus Cell Brush Border Membrane Sodium-Bile Acid Cotransport.
Cellular polarity modulates drug resistance in primary colorectal cancers via orientation of the multidrug resistance protein ABCB1.
Inhibition of ezrin causes PKCα-mediated internalization of erbb2/HER2 tyrosine kinase in breast cancer cells.
Activated ezrin controls MISP levels to ensure correct NuMA polarization and spindle orientation.
Suppressor of IKKepsilon forms direct interactions with cytoskeletal proteins, tubulin and α-actinin, linking innate immunity to the cytoskeleton.
Distinct Ezrin Truncations Differentiate Metastases in Sentinel Lymph Nodes from Unaffected Lymph Node Tissues, from Primary Breast Tumors, and from Healthy Glandular Breast Tissues.
Prognostic significance of CD133 and ezrin expression in colorectal carcinoma.
Molecular dissection of colorectal cancer in pre-clinical models identifies biomarkers predicting sensitivity to EGFR inhibitors.
CPI-17 drives oncogenic Ras signaling in human melanomas via Ezrin-Radixin-Moesin family proteins.
The adenosine A2B receptor is involved in anion secretion in human pancreatic duct Capan-1 epithelial cells.
Tumor Suppressor NF2 Blocks Cellular Migration by Inhibiting Ectodomain Cleavage of CD44.
A three-protein signature and clinical outcome in esophageal squamous cell carcinoma.
Are podoplanin and ezrin involved in the invasion process of the ameloblastomas?
Transcription factors SOX4 and SOX9 cooperatively control development of bile ducts.
Decreased ezrin and paxillin expression in human urothelial bladder tumors correlate with tumor progression.
Fascin-1, ezrin and paxillin contribute to the malignant progression and are predictors of clinical prognosis in laryngeal squamous cell carcinoma.
Cytoplasmic ezrin and moesin correlate with poor survival in head and neck squamous cell carcinoma.
Phosphorylation of EBP50 negatively regulates β-PIX-dependent Rac1 activity in anoikis.
Role of CD44 in the organization of keratinocyte pericellular hyaluronan.
Role of the ductal transcription factors HNF6 and Sox9 in pancreatic acinar-to-ductal metaplasia.
Activation of Ras requires the ERM-dependent link of actin to the plasma membrane.
Sequential dose-dense Doxorubicin and Ifosfamide in advanced soft-tissue sarcoma patients in an out-patient-basis schedule.
Expression of ezrin in human embryonic, fetal, and normal adult tissues.
Phosphorylation of podocalyxin (Ser415) Prevents RhoA and ezrin activation and disrupts its interaction with the actin cytoskeleton.
ERM stable knockdown by siRNA reduced in vitro migration and invasion of human SGC-7901 cells.
Lipid rafts and cytoskeletal proteins in placental microvilli membranes from preeclamptic and IUGR pregnancies.
Cell growing density affects the structural and functional properties of Caco-2 differentiated monolayer.
Expression of the membrane-cytoskeletal linker Ezrin in salivary gland adenoid cystic carcinoma.
Ezrin-mediated apical integrity is required for intestinal homeostasis.
The effect of sphingomyelin synthase 2 (SMS2) deficiency on the expression of drug transporters in mouse brain.
c-Met recruits ICAM-1 as a coreceptor to compensate for the loss of CD44 in Cd44 null mice.
Podocyte membrane vesicles in urine originate from tip vesiculation of podocyte microvilli.
p35 is required for CDK5 activation in cellular senescence.
Expression and clinical significance of Ezrin and E-cadherin in esophageal squamous cell carcinoma.
Expression and clinical significance of Ezrin and E-cadherin in esophageal squamous cell carcinoma.
Ezrin mediates c-Myc actions in prostate cancer cell invasion.
Ezrin is highly expressed in early thymocytes, but dispensable for T cell development in mice.
Immunohistochemical profiling of node negative breast carcinomas allows prediction of metastatic risk.
Immunohistochemical profiling of node negative breast carcinomas allows prediction of metastatic risk.
A signature predictive of disease outcome in breast carcinomas, identified by quantitative immunocytochemical assays.
Establishment and characterization of 4 new human pancreatic cancer cell lines: evidences of different tumor phenotypes.
Ezrin-radixin-moesin-binding phosphoprotein (EBP50), an estrogen-inducible scaffold protein, contributes to biliary epithelial cell proliferation.
Involvement of CD44v6 in InlB-dependent Listeria invasion.
Aberrant epithelial morphology and persistent epidermal growth factor receptor signaling in a mouse model of renal carcinoma.
Slit diaphragms contain tight junction proteins.
Expression of ezrin is associated with invasion and dedifferentiation of hepatitis B related hepatocellular carcinoma.
Quantitative immunocytochemical profile to predict early outcome of disease in triple-negative breast carcinomas.
Quantitative immunocytochemical profile to predict early outcome of disease in triple-negative breast carcinomas.
Duox maturation factors form cell surface complexes with Duox affecting the specificity of reactive oxygen species generation.
Ezrin silencing by small hairpin RNA reverses metastatic behaviors of human breast cancer cells.
NK cell protease granzyme M targets alpha-tubulin and disorganizes the microtubule network.
NK cell protease granzyme M targets alpha-tubulin and disorganizes the microtubule network.
Nestin expression in repopulating mesangial cells promotes their proliferation.
Inhibition of T cell activation by cyclic adenosine 5'-monophosphate requires lipid raft targeting of protein kinase A type I by the A-kinase anchoring protein ezrin.
Adhesion molecules as prognostic markers in pancreatic adenocarcinoma.
Ezrin and radixin both regulate the apical membrane localization of ABCC2 (MRP2) in human intestinal epithelial Caco-2 cells.
A bipartite signal regulates the faithful delivery of apical domain marker podocalyxin/Gp135.
Association of ezrin expression in intestinal and diffuse gastric carcinoma with clinicopathological parameters and tumor type.
Association of ezrin expression in intestinal and diffuse gastric carcinoma with clinicopathological parameters and tumor type.
Ezrin in osteosarcoma: comparison between conventional high-grade and central low-grade osteosarcoma.
Androgen induction of prostate cancer cell invasion is mediated by ezrin.
Differential tissue and subcellular expressionof ERM proteins in normal and malignant tissues: cytoplasmic ezrin expression has prognostic signficance for head and neck squamous cell carcinoma.
Prognostic impact of immunohistochemical expression of ezrin in highly malignant soft tissue sarcomas.
Ezrin expression in prostate cancer and benign prostatic tissue.
Stromal cells in hemangioblastoma: neuroectodermal differentiation and morphological similarities to ependymoma.
Myocilin is expressed in the glomerulus of the kidney and induced in mesangioproliferative glomerulonephritis.
Expression of ezrin in prostatic intraepithelial neoplasia.
Ezrin is essential for epithelial organization and villus morphogenesis in the developing intestine.
Ezrin immunoreactivity in relation to survival in serous ovarian carcinoma patients.
Ezrin immunoreactivity in relation to survival in serous ovarian carcinoma patients.
Gene expression profiling of androgen deficiency predicts a pathway of prostate apoptosis that involves genes related to oxidative stress.
Thyroid hormone-deficient period prior to the onset of hearing is associated with reduced levels of beta-tectorin protein in the tectorial membrane: implication for hearing loss.
ERM-dependent movement of CD43 defines a novel protein complex distal to the immunological synapse.
Chen L, Perera ND, Karoukis AJ, Feathers KL, Ali RR, Thompson DA, Fahim AT
Scientific reports 2022 Jul 26;12(1):12694
Scientific reports 2022 Jul 26;12(1):12694
A Structurally Conserved RNA Element within SARS-CoV-2 ORF1a RNA and S mRNA Regulates Translation in Response to Viral S Protein-Induced Signaling in Human Lung Cells.
Basu A, Penumutchu S, Nguyen K, Mbonye U, Tolbert BS, Karn J, Komar AA, Mazumder B
Journal of virology 2022 Jan 26;96(2):e0167821
Journal of virology 2022 Jan 26;96(2):e0167821
Astrovirus-induced epithelial-mesenchymal transition via activated TGF-β increases viral replication.
Hargest V, Bub T, Neale G, Schultz-Cherry S
PLoS pathogens 2022 Apr;18(4):e1009716
PLoS pathogens 2022 Apr;18(4):e1009716
Role of FGF and Hyaluronan in Choroidal Neovascularization in Sorsby Fundus Dystrophy.
Wolk A, Hatipoglu D, Cutler A, Ali M, Bell L, Hua Qi J, Singh R, Batoki J, Karle L, Bonilha VL, Wessely O, Stoehr H, Hascall V, Anand-Apte B
Cells 2020 Mar 4;9(3)
Cells 2020 Mar 4;9(3)
Mechanism of Dyslipidemia in Obesity-Unique Regulation of Ileal Villus Cell Brush Border Membrane Sodium-Bile Acid Cotransport.
Sundaram S, Palaniappan B, Nepal N, Chaffins S, Sundaram U, Arthur S
Cells 2019 Oct 3;8(10)
Cells 2019 Oct 3;8(10)
Cellular polarity modulates drug resistance in primary colorectal cancers via orientation of the multidrug resistance protein ABCB1.
Ashley N, Ouaret D, Bodmer WF
The Journal of pathology 2019 Mar;247(3):293-304
The Journal of pathology 2019 Mar;247(3):293-304
Inhibition of ezrin causes PKCα-mediated internalization of erbb2/HER2 tyrosine kinase in breast cancer cells.
Jeong J, Choi J, Kim W, Dann P, Takyar F, Gefter JV, Friedman PA, Wysolmerski JJ
The Journal of biological chemistry 2019 Jan 18;294(3):887-901
The Journal of biological chemistry 2019 Jan 18;294(3):887-901
Activated ezrin controls MISP levels to ensure correct NuMA polarization and spindle orientation.
Kschonsak YT, Hoffmann I
Journal of cell science 2018 May 21;131(10)
Journal of cell science 2018 May 21;131(10)
Suppressor of IKKepsilon forms direct interactions with cytoskeletal proteins, tubulin and α-actinin, linking innate immunity to the cytoskeleton.
Sonnenschein HA, Lawrence KF, Wittenberg KA, Slykas FA, Dohleman EL, Knoublauch JB, Fahey SM, Marshall TM Jr, Marion JD, Bell JK
FEBS open bio 2018 Jul;8(7):1064-1082
FEBS open bio 2018 Jul;8(7):1064-1082
Distinct Ezrin Truncations Differentiate Metastases in Sentinel Lymph Nodes from Unaffected Lymph Node Tissues, from Primary Breast Tumors, and from Healthy Glandular Breast Tissues.
Röwer C, George C, Reimer T, Stengel B, Radtke A, Gerber B, Glocker MO
Translational oncology 2018 Feb;11(1):1-10
Translational oncology 2018 Feb;11(1):1-10
Prognostic significance of CD133 and ezrin expression in colorectal carcinoma.
Fathi A, Mosaad H, Hussein S, Roshdy M, Ismail EI
IUBMB life 2017 May;69(5):328-340
IUBMB life 2017 May;69(5):328-340
Molecular dissection of colorectal cancer in pre-clinical models identifies biomarkers predicting sensitivity to EGFR inhibitors.
Schütte M, Risch T, Abdavi-Azar N, Boehnke K, Schumacher D, Keil M, Yildiriman R, Jandrasits C, Borodina T, Amstislavskiy V, Worth CL, Schweiger C, Liebs S, Lange M, Warnatz HJ, Butcher LM, Barrett JE, Sultan M, Wierling C, Golob-Schwarzl N, Lax S, Uranitsch S, Becker M, Welte Y, Regan JL, Silvestrov M, Kehler I, Fusi A, Kessler T, Herwig R, Landegren U, Wienke D, Nilsson M, Velasco JA, Garin-Chesa P, Reinhard C, Beck S, Schäfer R, Regenbrecht CR, Henderson D, Lange B, Haybaeck J, Keilholz U, Hoffmann J, Lehrach H, Yaspo ML
Nature communications 2017 Feb 10;8:14262
Nature communications 2017 Feb 10;8:14262
CPI-17 drives oncogenic Ras signaling in human melanomas via Ezrin-Radixin-Moesin family proteins.
Riecken LB, Zoch A, Wiehl U, Reichert S, Scholl I, Cui Y, Ziemer M, Anderegg U, Hagel C, Morrison H
Oncotarget 2016 Nov 29;7(48):78242-78254
Oncotarget 2016 Nov 29;7(48):78242-78254
The adenosine A2B receptor is involved in anion secretion in human pancreatic duct Capan-1 epithelial cells.
Hayashi M, Inagaki A, Novak I, Matsuda H
Pflugers Archiv : European journal of physiology 2016 Jul;468(7):1171-1181
Pflugers Archiv : European journal of physiology 2016 Jul;468(7):1171-1181
Tumor Suppressor NF2 Blocks Cellular Migration by Inhibiting Ectodomain Cleavage of CD44.
Hartmann M, Parra LM, Ruschel A, Böhme S, Li Y, Morrison H, Herrlich A, Herrlich P
Molecular cancer research : MCR 2015 May;13(5):879-90
Molecular cancer research : MCR 2015 May;13(5):879-90
A three-protein signature and clinical outcome in esophageal squamous cell carcinoma.
Cao HH, Zhang SY, Shen JH, Wu ZY, Wu JY, Wang SH, Li EM, Xu LY
Oncotarget 2015 Mar 10;6(7):5435-48
Oncotarget 2015 Mar 10;6(7):5435-48
Are podoplanin and ezrin involved in the invasion process of the ameloblastomas?
Costa YF, Tjioe KC, Nonogaki S, Soares FA, Lauris JR, Oliveira DT
European journal of histochemistry : EJH 2015 Feb 24;59(1):2451
European journal of histochemistry : EJH 2015 Feb 24;59(1):2451
Transcription factors SOX4 and SOX9 cooperatively control development of bile ducts.
Poncy A, Antoniou A, Cordi S, Pierreux CE, Jacquemin P, Lemaigre FP
Developmental biology 2015 Aug 15;404(2):136-48
Developmental biology 2015 Aug 15;404(2):136-48
Decreased ezrin and paxillin expression in human urothelial bladder tumors correlate with tumor progression.
Athanasopoulou A, Aroukatos P, Nakas D, Repanti M, Papadaki H, Bravou V
Urologic oncology 2013 Aug;31(6):836-42
Urologic oncology 2013 Aug;31(6):836-42
Fascin-1, ezrin and paxillin contribute to the malignant progression and are predictors of clinical prognosis in laryngeal squamous cell carcinoma.
Gao W, Zhang C, Feng Y, Chen G, Wen S, Huangfu H, Wang B
PloS one 2012;7(11):e50710
PloS one 2012;7(11):e50710
Cytoplasmic ezrin and moesin correlate with poor survival in head and neck squamous cell carcinoma.
Schlecht NF, Brandwein-Gensler M, Smith RV, Kawachi N, Broughel D, Lin J, Keller CE, Reynolds PA, Gunn-Moore FJ, Harris T, Childs G, Belbin TJ, Prystowsky MB
Head and neck pathology 2012 Jun;6(2):232-43
Head and neck pathology 2012 Jun;6(2):232-43
Phosphorylation of EBP50 negatively regulates β-PIX-dependent Rac1 activity in anoikis.
Chen JY, Lin YY, Jou TS
Cell death and differentiation 2012 Jun;19(6):1027-37
Cell death and differentiation 2012 Jun;19(6):1027-37
Role of CD44 in the organization of keratinocyte pericellular hyaluronan.
Pasonen-Seppänen S, Hyttinen JM, Rilla K, Jokela T, Noble PW, Tammi M, Tammi R
Histochemistry and cell biology 2012 Jan;137(1):107-20
Histochemistry and cell biology 2012 Jan;137(1):107-20
Role of the ductal transcription factors HNF6 and Sox9 in pancreatic acinar-to-ductal metaplasia.
Prévot PP, Simion A, Grimont A, Colletti M, Khalaileh A, Van den Steen G, Sempoux C, Xu X, Roelants V, Hald J, Bertrand L, Heimberg H, Konieczny SF, Dor Y, Lemaigre FP, Jacquemin P
Gut 2012 Dec;61(12):1723-32
Gut 2012 Dec;61(12):1723-32
Activation of Ras requires the ERM-dependent link of actin to the plasma membrane.
Sperka T, Geissler KJ, Merkel U, Scholl I, Rubio I, Herrlich P, Morrison HL
PloS one 2011;6(11):e27511
PloS one 2011;6(11):e27511
Sequential dose-dense Doxorubicin and Ifosfamide in advanced soft-tissue sarcoma patients in an out-patient-basis schedule.
Almeida GF, Castro G Jr, Snitcovsky IM, Siqueira SA, Akaishi EH, Camargo OP, Oliveira CR, Federico MH
Sarcoma 2011;2011:984340
Sarcoma 2011;2011:984340
Expression of ezrin in human embryonic, fetal, and normal adult tissues.
Xie JJ, Zhang FR, Tao LH, Lü Z, Xu XE, Jian-Shen, Xu LY, Li EM
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2011 Nov;59(11):1001-8
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2011 Nov;59(11):1001-8
Phosphorylation of podocalyxin (Ser415) Prevents RhoA and ezrin activation and disrupts its interaction with the actin cytoskeleton.
Fukasawa H, Obayashi H, Schmieder S, Lee J, Ghosh P, Farquhar MG
The American journal of pathology 2011 Nov;179(5):2254-65
The American journal of pathology 2011 Nov;179(5):2254-65
ERM stable knockdown by siRNA reduced in vitro migration and invasion of human SGC-7901 cells.
Ou-Yang M, Liu HR, Zhang Y, Zhu X, Yang Q
Biochimie 2011 May;93(5):954-61
Biochimie 2011 May;93(5):954-61
Lipid rafts and cytoskeletal proteins in placental microvilli membranes from preeclamptic and IUGR pregnancies.
Riquelme G, Vallejos C, de Gregorio N, Morales B, Godoy V, Berrios M, Bastías N, Rodríguez C
The Journal of membrane biology 2011 Jun;241(3):127-40
The Journal of membrane biology 2011 Jun;241(3):127-40
Cell growing density affects the structural and functional properties of Caco-2 differentiated monolayer.
Natoli M, Leoni BD, D'Agnano I, D'Onofrio M, Brandi R, Arisi I, Zucco F, Felsani A
Journal of cellular physiology 2011 Jun;226(6):1531-43
Journal of cellular physiology 2011 Jun;226(6):1531-43
Expression of the membrane-cytoskeletal linker Ezrin in salivary gland adenoid cystic carcinoma.
Wang YY, Chen WL, Huang ZQ, Yang ZH, Zhang B, Wang JG, Li HG, Li JS
Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics 2011 Jul;112(1):96-104
Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics 2011 Jul;112(1):96-104
Ezrin-mediated apical integrity is required for intestinal homeostasis.
Casaletto JB, Saotome I, Curto M, McClatchey AI
Proceedings of the National Academy of Sciences of the United States of America 2011 Jul 19;108(29):11924-9
Proceedings of the National Academy of Sciences of the United States of America 2011 Jul 19;108(29):11924-9
The effect of sphingomyelin synthase 2 (SMS2) deficiency on the expression of drug transporters in mouse brain.
Zhang Y, Dong J, Zhu X, Wang W, Yang Q
Biochemical pharmacology 2011 Aug 1;82(3):287-94
Biochemical pharmacology 2011 Aug 1;82(3):287-94
c-Met recruits ICAM-1 as a coreceptor to compensate for the loss of CD44 in Cd44 null mice.
Olaku V, Matzke A, Mitchell C, Hasenauer S, Sakkaravarthi A, Pace G, Ponta H, Orian-Rousseau V
Molecular biology of the cell 2011 Aug 1;22(15):2777-86
Molecular biology of the cell 2011 Aug 1;22(15):2777-86
Podocyte membrane vesicles in urine originate from tip vesiculation of podocyte microvilli.
Hara M, Yanagihara T, Hirayama Y, Ogasawara S, Kurosawa H, Sekine S, Kihara I
Human pathology 2010 Sep;41(9):1265-75
Human pathology 2010 Sep;41(9):1265-75
p35 is required for CDK5 activation in cellular senescence.
Mao D, Hinds PW
The Journal of biological chemistry 2010 May 7;285(19):14671-80
The Journal of biological chemistry 2010 May 7;285(19):14671-80
Expression and clinical significance of Ezrin and E-cadherin in esophageal squamous cell carcinoma.
Zhai JW, Yang XG, Yang FS, Hu JG, Hua WX
Chinese journal of cancer 2010 Mar;29(3):317-20
Chinese journal of cancer 2010 Mar;29(3):317-20
Expression and clinical significance of Ezrin and E-cadherin in esophageal squamous cell carcinoma.
Zhai JW, Yang XG, Yang FS, Hu JG, Hua WX
Chinese journal of cancer 2010 Mar;29(3):317-20
Chinese journal of cancer 2010 Mar;29(3):317-20
Ezrin mediates c-Myc actions in prostate cancer cell invasion.
Chuan YC, Iglesias-Gato D, Fernandez-Perez L, Cedazo-Minguez A, Pang ST, Norstedt G, Pousette A, Flores-Morales A
Oncogene 2010 Mar 11;29(10):1531-42
Oncogene 2010 Mar 11;29(10):1531-42
Ezrin is highly expressed in early thymocytes, but dispensable for T cell development in mice.
Shaffer MH, Huang Y, Corbo E, Wu GF, Velez M, Choi JK, Saotome I, Cannon JL, McClatchey AI, Sperling AI, Maltzman JS, Oliver PM, Bhandoola A, Laufer TM, Burkhardt JK
PloS one 2010 Aug 27;5(8):e12404
PloS one 2010 Aug 27;5(8):e12404
Immunohistochemical profiling of node negative breast carcinomas allows prediction of metastatic risk.
Giusiano S, Secq V, Carcopino X, Carpentier S, Andrac L, Lavaut MN, Allasia C, Bonnier P, Iovanna JL, Garcia S, Boubli L, Birnbaum D, Charpin C
International journal of oncology 2010 Apr;36(4):889-98
International journal of oncology 2010 Apr;36(4):889-98
Immunohistochemical profiling of node negative breast carcinomas allows prediction of metastatic risk.
Giusiano S, Secq V, Carcopino X, Carpentier S, Andrac L, Lavaut MN, Allasia C, Bonnier P, Iovanna JL, Garcia S, Boubli L, Birnbaum D, Charpin C
International journal of oncology 2010 Apr;36(4):889-98
International journal of oncology 2010 Apr;36(4):889-98
A signature predictive of disease outcome in breast carcinomas, identified by quantitative immunocytochemical assays.
Charpin C, Secq V, Giusiano S, Carpentier S, Andrac L, Lavaut MN, Allasia C, Bonnier P, Garcia S
International journal of cancer 2009 May 1;124(9):2124-34
International journal of cancer 2009 May 1;124(9):2124-34
Establishment and characterization of 4 new human pancreatic cancer cell lines: evidences of different tumor phenotypes.
Chifenti B, Morelli M, Zavaglia M, Coviello DA, Guerneri S, Santucci A, Paffetti A, Masetti M, Locci MT, Bertacca G, Capodanno A, Collecchi P, Campani D, Mosca F, Bevilacqua G, Cavazzana AO
Pancreas 2009 Mar;38(2):184-96
Pancreas 2009 Mar;38(2):184-96
Ezrin-radixin-moesin-binding phosphoprotein (EBP50), an estrogen-inducible scaffold protein, contributes to biliary epithelial cell proliferation.
Fouassier L, Rosenberg P, Mergey M, Saubaméa B, Clapéron A, Kinnman N, Chignard N, Jacobsson-Ekman G, Strandvik B, Rey C, Barbu V, Hultcrantz R, Housset C
The American journal of pathology 2009 Mar;174(3):869-80
The American journal of pathology 2009 Mar;174(3):869-80
Involvement of CD44v6 in InlB-dependent Listeria invasion.
Jung C, Matzke A, Niemann HH, Schwerk C, Tenenbaum T, Orian-Rousseau V
Molecular microbiology 2009 Jun;72(5):1196-207
Molecular microbiology 2009 Jun;72(5):1196-207
Aberrant epithelial morphology and persistent epidermal growth factor receptor signaling in a mouse model of renal carcinoma.
Morris ZS, McClatchey AI
Proceedings of the National Academy of Sciences of the United States of America 2009 Jun 16;106(24):9767-72
Proceedings of the National Academy of Sciences of the United States of America 2009 Jun 16;106(24):9767-72
Slit diaphragms contain tight junction proteins.
Fukasawa H, Bornheimer S, Kudlicka K, Farquhar MG
Journal of the American Society of Nephrology : JASN 2009 Jul;20(7):1491-503
Journal of the American Society of Nephrology : JASN 2009 Jul;20(7):1491-503
Expression of ezrin is associated with invasion and dedifferentiation of hepatitis B related hepatocellular carcinoma.
Yeh CN, Pang ST, Chen TW, Wu RC, Weng WH, Chen MF
BMC cancer 2009 Jul 15;9:233
BMC cancer 2009 Jul 15;9:233
Quantitative immunocytochemical profile to predict early outcome of disease in triple-negative breast carcinomas.
Charpin C, Giusiano S, Secq V, Carpentier S, Andrac L, Lavaut MN, Allasia C, Bonnier P, Garcia S
International journal of oncology 2009 Apr;34(4):983-93
International journal of oncology 2009 Apr;34(4):983-93
Quantitative immunocytochemical profile to predict early outcome of disease in triple-negative breast carcinomas.
Charpin C, Giusiano S, Secq V, Carpentier S, Andrac L, Lavaut MN, Allasia C, Bonnier P, Garcia S
International journal of oncology 2009 Apr;34(4):983-93
International journal of oncology 2009 Apr;34(4):983-93
Duox maturation factors form cell surface complexes with Duox affecting the specificity of reactive oxygen species generation.
Morand S, Ueyama T, Tsujibe S, Saito N, Korzeniowska A, Leto TL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2009 Apr;23(4):1205-18
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2009 Apr;23(4):1205-18
Ezrin silencing by small hairpin RNA reverses metastatic behaviors of human breast cancer cells.
Li Q, Wu M, Wang H, Xu G, Zhu T, Zhang Y, Liu P, Song A, Gang C, Han Z, Zhou J, Meng L, Lu Y, Wang S, Ma D
Cancer letters 2008 Mar 8;261(1):55-63
Cancer letters 2008 Mar 8;261(1):55-63
NK cell protease granzyme M targets alpha-tubulin and disorganizes the microtubule network.
Bovenschen N, de Koning PJ, Quadir R, Broekhuizen R, Damen JM, Froelich CJ, Slijper M, Kummer JA
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 15;180(12):8184-91
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 15;180(12):8184-91
NK cell protease granzyme M targets alpha-tubulin and disorganizes the microtubule network.
Bovenschen N, de Koning PJ, Quadir R, Broekhuizen R, Damen JM, Froelich CJ, Slijper M, Kummer JA
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 15;180(12):8184-91
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 15;180(12):8184-91
Nestin expression in repopulating mesangial cells promotes their proliferation.
Daniel C, Albrecht H, Lüdke A, Hugo C
Laboratory investigation; a journal of technical methods and pathology 2008 Apr;88(4):387-97
Laboratory investigation; a journal of technical methods and pathology 2008 Apr;88(4):387-97
Inhibition of T cell activation by cyclic adenosine 5'-monophosphate requires lipid raft targeting of protein kinase A type I by the A-kinase anchoring protein ezrin.
Ruppelt A, Mosenden R, Grönholm M, Aandahl EM, Tobin D, Carlson CR, Abrahamsen H, Herberg FW, Carpén O, Taskén K
Journal of immunology (Baltimore, Md. : 1950) 2007 Oct 15;179(8):5159-68
Journal of immunology (Baltimore, Md. : 1950) 2007 Oct 15;179(8):5159-68
Adhesion molecules as prognostic markers in pancreatic adenocarcinoma.
Torer N, Kayaselcuk F, Nursal TZ, Yildirim S, Tarim A, Nòyan T, Karakayali H
Journal of surgical oncology 2007 Oct 1;96(5):419-23
Journal of surgical oncology 2007 Oct 1;96(5):419-23
Ezrin and radixin both regulate the apical membrane localization of ABCC2 (MRP2) in human intestinal epithelial Caco-2 cells.
Yang Q, Onuki R, Nakai C, Sugiyama Y
Experimental cell research 2007 Oct 1;313(16):3517-25
Experimental cell research 2007 Oct 1;313(16):3517-25
A bipartite signal regulates the faithful delivery of apical domain marker podocalyxin/Gp135.
Yu CY, Chen JY, Lin YY, Shen KF, Lin WL, Chien CL, ter Beest MB, Jou TS
Molecular biology of the cell 2007 May;18(5):1710-22
Molecular biology of the cell 2007 May;18(5):1710-22
Association of ezrin expression in intestinal and diffuse gastric carcinoma with clinicopathological parameters and tumor type.
Bal N, Yildirim S, Nursal TZ, Bolat F, Kayaselcuk F
World journal of gastroenterology 2007 Jul 21;13(27):3726-9
World journal of gastroenterology 2007 Jul 21;13(27):3726-9
Association of ezrin expression in intestinal and diffuse gastric carcinoma with clinicopathological parameters and tumor type.
Bal N, Yildirim S, Nursal TZ, Bolat F, Kayaselcuk F
World journal of gastroenterology 2007 Jul 21;13(27):3726-9
World journal of gastroenterology 2007 Jul 21;13(27):3726-9
Ezrin in osteosarcoma: comparison between conventional high-grade and central low-grade osteosarcoma.
Park HR, Jung WW, Bacchini P, Bertoni F, Kim YW, Park YK
Pathology, research and practice 2006;202(7):509-15
Pathology, research and practice 2006;202(7):509-15
Androgen induction of prostate cancer cell invasion is mediated by ezrin.
Chuan YC, Pang ST, Cedazo-Minguez A, Norstedt G, Pousette A, Flores-Morales A
The Journal of biological chemistry 2006 Oct 6;281(40):29938-48
The Journal of biological chemistry 2006 Oct 6;281(40):29938-48
Differential tissue and subcellular expressionof ERM proteins in normal and malignant tissues: cytoplasmic ezrin expression has prognostic signficance for head and neck squamous cell carcinoma.
Madan R, Brandwein-Gensler M, Schlecht NF, Elias K, Gorbovitsky E, Belbin TJ, Mahmood R, Breining D, Qian H, Childs G, Locker J, Smith R, Haigentz M Jr, Gunn-Moore F, Prystowsky MB
Head & neck 2006 Nov;28(11):1018-27
Head & neck 2006 Nov;28(11):1018-27
Prognostic impact of immunohistochemical expression of ezrin in highly malignant soft tissue sarcomas.
Weng WH, Ahlén J, Aström K, Lui WO, Larsson C
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Sep 1;11(17):6198-204
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Sep 1;11(17):6198-204
Ezrin expression in prostate cancer and benign prostatic tissue.
Valdman A, Fang X, Pang ST, Nilsson B, Ekman P, Egevad L
European urology 2005 Nov;48(5):852-7
European urology 2005 Nov;48(5):852-7
Stromal cells in hemangioblastoma: neuroectodermal differentiation and morphological similarities to ependymoma.
Ishizawa K, Komori T, Hirose T
Pathology international 2005 Jul;55(7):377-85
Pathology international 2005 Jul;55(7):377-85
Myocilin is expressed in the glomerulus of the kidney and induced in mesangioproliferative glomerulonephritis.
Goldwich A, Baulmann DC, Ohlmann A, Flügel-Koch C, Schöcklmann H, Tamm ER
Kidney international 2005 Jan;67(1):140-51
Kidney international 2005 Jan;67(1):140-51
Expression of ezrin in prostatic intraepithelial neoplasia.
Pang ST, Fang X, Valdman A, Norstedt G, Pousette A, Egevad L, Ekman P
Urology 2004 Mar;63(3):609-12
Urology 2004 Mar;63(3):609-12
Ezrin is essential for epithelial organization and villus morphogenesis in the developing intestine.
Saotome I, Curto M, McClatchey AI
Developmental cell 2004 Jun;6(6):855-64
Developmental cell 2004 Jun;6(6):855-64
Ezrin immunoreactivity in relation to survival in serous ovarian carcinoma patients.
Moilanen J, Lassus H, Leminen A, Vaheri A, Bützow R, Carpén O
Gynecologic oncology 2003 Aug;90(2):273-81
Gynecologic oncology 2003 Aug;90(2):273-81
Ezrin immunoreactivity in relation to survival in serous ovarian carcinoma patients.
Moilanen J, Lassus H, Leminen A, Vaheri A, Bützow R, Carpén O
Gynecologic oncology 2003 Aug;90(2):273-81
Gynecologic oncology 2003 Aug;90(2):273-81
Gene expression profiling of androgen deficiency predicts a pathway of prostate apoptosis that involves genes related to oxidative stress.
Pang ST, Dillner K, Wu X, Pousette A, Norstedt G, Flores-Morales A
Endocrinology 2002 Dec;143(12):4897-906
Endocrinology 2002 Dec;143(12):4897-906
Thyroid hormone-deficient period prior to the onset of hearing is associated with reduced levels of beta-tectorin protein in the tectorial membrane: implication for hearing loss.
Knipper M, Richardson G, Mack A, Müller M, Goodyear R, Limberger A, Rohbock K, Köpschall I, Zenner HP, Zimmermann U
The Journal of biological chemistry 2001 Oct 19;276(42):39046-52
The Journal of biological chemistry 2001 Oct 19;276(42):39046-52
ERM-dependent movement of CD43 defines a novel protein complex distal to the immunological synapse.
Allenspach EJ, Cullinan P, Tong J, Tang Q, Tesciuba AG, Cannon JL, Takahashi SM, Morgan R, Burkhardt JK, Sperling AI
Immunity 2001 Nov;15(5):739-50
Immunity 2001 Nov;15(5):739-50
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Supportive validation
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- Western blot analysis of VIL2 was performed with 10 µg of HeLa cells transfected with Transfection Reagent alone (Lane 1), 100nM Non-Targeting control siRNA (Lane 2), or 100nM siRNA against VIL2 (Lane 3). Proteins were resolved using a NuPAGE® Novex 4-12% Bis-Tris Gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), and a protein size ladder. Proteins were wet transferred to a Pierce Nitrocellulose Membrane (Product # 88025) OR Pierce PVDF Membrane (Product # 88518) and blocked with Pierce Starting Block T20 (PBS) Blocking Buffer (Product # 37539) for 1 hour at room temperature. VIL2 was detected at ~ 81 kDa using VIL2 Mouse monoclonal antibody (Product # MA5-13862) diluted in Pierce Starting Block T20 (PBS) Blocking Buffer 4°C overnight on a rocking platform. Pierce Goat Anti-Mouse (Product # 31437) HRP-Conjugated Antibodies at a 1:2500 dilution were used and chemiluminescent detection was performed using Pierce Supersignal West Dura Maximum Sensitivity Substrate (Product # 37071). Relative density of the bands normalized to GAPDH (36 kDa). VIL2 Antibody (Product # MA5-13862) confirms silencing of VIL2 expression.
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- CRISPR-Cas9 mediated genome editing ofEzrin (as confirmed by next generation sequencing) was achieved by using LentiArray™ Lentiviral sgRNA (Product # A32042, AssayID CRISPR864181_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Fig (a) Western blot analysis of Ezrin was performed by loading 30 µg of HeLa Wild Type (Lane 1), HeLa Cas9 (Lane 2) and HeLa Cas9 cells transduced with Ezrin Lentiviral sgRNA (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with Anti-Ezrin Monoclonal Antibody (3C12) (Product # MA5-13862) using 0.5 µg/mL dilution and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177 1:5,000 dilution).Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Loss of signal in sgRNA transduced cells using the LentiArray™ CRISPR product line confirms that antibody is specific toEzrin (Fig (b)).
Supportive validation
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- Immunocytochemistry analysis of Ezrin in U2OS cells. Cells fixed with 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 (Product # 28314) in PBS for 15 minutes at room temperature and blocked with 2% BSA in PBST (Product # 37525) for 30 minutes at room temperature. Cells were treated with Peroxidase Suppressor (Product # 35000), probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBST, and incubated with an HRP-conjugated goat anti-mouse IgG (H+L) secondary antibody (Product # 31431) at a dilution of 1:1000 for 30 minutes at room temperature. Chromogenic detection was performed using Metal Enhanced DAB Substrate Kit (Product # 34065). Images were taken on a Zeiss Axio Observer microscope at 20X magnification (x1.6 Optovar ~ 32X).
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- Immunocytochemistry analysis of Ezrin in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 (Product # 28314) in TBST (Product # 28376) for 30 minutes at room temperature and blocked with Universal Blocker (from Product # 03-6000) for 30 minutes at room temperature. Cells were probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBST, and incubated with an alkaline phosphatase-conjugated goat anti-mouse IgG (H+L) secondary antibody (Product # 31320) at a dilution of 1:250 for 30 minutes at room temperature. Chromogenic detection was performed using 1-Step NBT/BCIP (Product # 34042). Images were taken on a Zeiss Axio Observer microscope at 20X magnification (x1.6 Optovar ~ 32X).
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- Immunofluorescent analysis of Phalloidin (orange) and Ezrin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/mL final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1 µg/mL for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
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- Immunofluorescent analysis of Phalloidin (magenta) and Ezrin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 633 Phalloidin (Product # 21840) at a dilution of 1:120 (2.5units/mL final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1 µg/mL for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
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- Immunofluorescent analysis of Phalloidin (dark red) and Ezrin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 680 Phalloidin (Product # 21839) at a dilution of 1:120 (2.5 units/mL final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1 µg/mL for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
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- Immunohistochemistry was performed on paraffin-embedded human breast cancer tissue sections. To expose target proteins, heat-induced epitope retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 20 minutes at 95ºC. Following antigen retrieval, tissues were blocked in 3% BSA (Product # 37525) in PBST for 30 minutes at room temperature and then probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a dilution of 1:100 for 1 hour in a humidified chamber. Tissues were washed extensively with PBS/0.025% Tween-20 (Product # 28314) and endogenous peroxidase activity quenched with Peroxidase Suppressor (Product # 35000) for 30 minutes at room temperature. Detection was performed using an HRP-conjugated goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:500 followed by colorimetric detection using Metal Enhanced DAB Substrate Kit (Product # 34065). Images were taken on a Zeiss Axiovision microscope at 40X magnification.
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- Formalin-fixed, paraffin-embedded human lung stained with Ezrin antibody using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of bronchio-epithelial cells.
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- Immunohistochemistry was performed on paraffin-embedded human colon cancer tissue sections. To expose target proteins, heat-induced epitope retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 20 minutes at 95ºC. Following antigen retrieval, tissues were blocked in 3% BSA (Product # 37525) in PBST for 30 minutes at room temperature and then probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a dilution of 1:100 for 1 hour in a humidified chamber. Tissues were washed extensively with PBS/0.025% Tween-20 (Product # 28314) and endogenous peroxidase activity quenched with Peroxidase Suppressor (Product # 35000) for 30 minutes at room temperature. Detection was performed using an HRP-conjugated goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:500 followed by colorimetric detection using Metal Enhanced DAB Substrate Kit (Product # 34065). Images were taken on a Zeiss Axiovision microscope at 40X magnification.
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- Immunoprecipitation of Ezrin using Ezrin Monoclonal Antibody (Product # MA5-13862) on Native Human Raji Cells.
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- Immunoprecipitation of Ezrin using Ezrin Monoclonal Antibody (Product # MA5-13862) on denatured Human Raji Cells.
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- Figure 2 HA is increased in circulation and in the RPE and choroid in mouse models of Sorsby''s fundus dystrophy. ( A ) HA was increased in plasma from S179C-TIMP3 knockin mice (Timp3 S179C6/S179C ) and TIMP3-KO (Timp3 -/- ) mice compared to wild-type (WT) littermates. (n >= 5). Data are presented as mean +- SEM. ( B ) Mouse retina sections stained with biotinylated HA binding protein (HABP) in the absence (upper panel) or presence (lower panel) of hyaluronidase to detect HA. HABP staining is specific for HA as shown by the absence of staining in sections treated with hyaluronidase (lower panel). Green: HA; blue: DAPI. ( C - H ) Representative images of HA staining of mouse sections from wild-type (WT) mice ( C , F ), S179C-TIMP3 mutant mice ( D , G ) and TIMP3-KO mice ( E , H ). HA is increased in the RPE and choroid of S179C-TIMP3 ( D ) and TIMP3-KO ( E ) mice compared to wild-type (WT) littermate controls ( C ). HA (green) is predominantly localized to the basal surface of the RPE (**) ( F - H ) and not to the apical surface (*) as shown by co-staining with ezrin, a marker for the apical microvilli of RPE (red). 40x images ( C - E ); 63x images ( F - H ). n >= 3 for all immunohistochemistry data.
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- Figure 7 In epithelial cells, SIKE colocalizes with actin cytoskeletal markers. DOV 13 cells were fixed, permeabilized, and stained with mouse anti-cytoskeletal marker antibody as indicated followed by anti-mouse Alexa Fluor 555 (red), rabbit anti- SIKE antibody followed by anti-rabbit Alexa Fluor 488 (green) and Hoechst 33342 (blue). F-actin was stained with Alexa Fluor 555 Phalloidin. Cytoskeletal markers: actin, alpha-actinin, alpha-tubulin, ezrin, myosin light chain 2 ( MLC 2), focal adhesion kinase ( FAK ), beta-catenin. Images are maximum intensity of z -stack projections. Scale bar, 10 mum. Images are representative of two independent experiments, five images/experiment. Images are maximum intensity z -stack projections. Intersection of fluorescence for SIKE to target was significant (>= 0.5) for actin, alpha-actinin, and ezrin.
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- Figure 8 In myeloid cells, SIKE colocalizes with actin and microtubule markers. RAW 264.7 cells were fixed, permeabilized, and stained with mouse anti-cytoskeletal marker antibody as indicated followed by anti-mouse Alexa Fluor 555 (red), rabbit anti- SIKE antibody followed by anti-rabbit Alexa Fluor 488 (green) and Hoechst 33342 (blue). F-actin was stained with Alexa Fluor 555 Phalloidin. Cytoskeletal markers: actin, alpha-actinin, alpha-tubulin, ezrin, MLC 2, focal adhesion kinase ( FAK ), beta-catenin. Images are maximum intensity of z -stack projections. Scale bar, 10 mum. Images are representative of two independent experiments, five images/experiment. Images are maximum intensity z -stack projections. Intersection of fluorescence for SIKE to target was significant (>= 0.5) for alpha-actinin.
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- Figure 9 Quantitation of colocalization between SIKE and cytoskeletal markers. volocity software (PerkinElmer) was used to define SIKE or cytoskeletal marker volumes on thresholded images. Fraction of intersecting volumes was determined for SIKE to marker compared to total SIKE volume and marker to SIKE compared to total marker volume (A, C). These graphs represent co-occurrence. Significant co-occurrence was noted when values were > 0.5 with a 95% confidence interval. Thresholded PCC was determined (B, D) to assess codistribution of SIKE with cytoskeletal markers. Error bars represent standard error, n = 5. (A) In epithelial cells, co-occurrence of actin, alpha-actinin, and ezrin was observed for SIKE fluorescence. In myeloid cells, co-occurrence of alpha-actinin was observed for SIKE fluorescence. No significant (>= 0.5, as noted in Ref. 27 ) codistribution of cytoskeletal marker and SIKE fluorescence was observed for either epithelial (B) or myeloid (D) cells.
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- 10.1371/journal.ppat.1009716.g003 Fig 3 HAstV-1 infection leads to a disruption of cellular polarity. (A) Caco-2 infected with HAstV-1 or mock infected (as indicated). Cells were fixed at 24 hpi in 100% ice-cold methanol and then stained for ezrin (red), sodium-potassium ATPase (green), and DAPI (blue). Images are representative of at least three independent experiments. (B) Basal or apical localization of sodium-potassium ATPase was determined by measuring intensity above and below cell midline of HAstV- or mock-infected cells using ImageJ. Error bars indicate standard deviations of three independent experiments performed in triplicate, and asterisks show statistical significance as measured by the two-tailed Student t test as follows: ***, P < 0.001.