PA5-63674

antibody from Invitrogen Antibodies

Targeting: CYP27A1 CP27, CTX, CYP27

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-63674

Provider

Invitrogen Antibodies

Product name

CYP27A1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: RQRSLEEIPR LGQLRFFFQL FVQGYALQLH QLQVLYKAKY GPMWMSYLGP QMHVNLASAP LLEQVMRQEG KYPVRNDMEL WKEHRDQHDL TYGPFTTEGH HWYQLRQALN QRLLKPAEAA LYTDAFNEVI DDFMTRLD

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CYP27A1 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue. Samples were probed using a CYP27A1 Polyclonal Antibody (Product # PA5-63674).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of CYP27A1 in human cell line Hep G2 using a CYP27A1 Polyclonal Antibody (Product # PA5-63674) shows localization to mitochondria.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CYP27A1 in human kidney tissue shows strong cytoplasmic positivity in cells in tubules. Samples were probed using a CYP27A1 Polyclonal Antibody (Product # PA5-63674).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CYP27A1 in human kidney and pancreas tissues using CYP27A1 Polyclonal Antibody (Product # PA5-63674). Corresponding CYP27A1 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CYP27A1 in human pancreas using CYP27A1 Polyclonal Antibody (Product # PA5-63674) shows low expression as expected.