PAB20912

antibody from Abnova Corporation

Targeting: HNRNPH2 FTP3, hnRNPH', HNRPH', HNRPH2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20912

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20912, RRID:AB_10962033

Product name

HNRNPH2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant HNRNPH2.

Antigen sequence

FIYTREGRPSGEAFVELESEEEVKLALKKDRETMG
HRYVEVFKSNSVEMDWVLKHTGPNSPDTANDGFVR
LRGLPFGCSKEEIVQFFSGLEIVPNGMTLPVDFQG
RSTGEAFVQFAS


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with HNRNPH2 polyclonal antibody (Cat # PAB20912) at 1:250-1:500 dilution.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of cell lysates with HNRNPH2 polyclonal antibody (Cat # PAB20912) at 1:250-1:500 dilution.Lane 1 : NIH/3T3Lane 2 : NBT-II

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with HNRNPH2 polyclonal antibody (Cat # PAB20912) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli, cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney with HNRNPH2 polyclonal antibody (Cat # PAB20912) shows nuclear positivity in both glomeruli and renal tubules at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)