RQ4519

antibody from NSJ Bioreagents

Targeting: COL4A1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ4519

Provider

NSJ Bioreagents

Product name

Collagen IV Antibody

Antibody type

Monoclonal

Description

Protein G purified

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

3G3

Vial size

100 µg

Storage

After reconstitution, the Collagen IV antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20deg;C. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HEK293T, 2) HeLa and 3) A549 cell lysate with Collagen IV antibody at 0.5ug/ml. Predicted molecular weight ~160 kDa but may be observed at a larger size due to glycosylation.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human lung cancer with Collagen IV antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human placenta with Collagen IV antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.