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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunohistochemistry [1]
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- Product number
- R1041B - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1041B, RRID:AB_978648
- Product name
- anti Collagen type IV
- Antibody type
- Polyclonal
- Antigen
- Collagen Type IV from Human and Bovine placenta.
- Reactivity
- Human, Bovine
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
Submitted references The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens.
Perlecan is critical for heart stability.
Behrens DT, Villone D, Koch M, Brunner G, Sorokin L, Robenek H, Bruckner-Tuderman L, Bruckner P, Hansen U
The Journal of biological chemistry 2012 May 25;287(22):18700-9
The Journal of biological chemistry 2012 May 25;287(22):18700-9
Perlecan is critical for heart stability.
Sasse P, Malan D, Fleischmann M, Roell W, Gustafsson E, Bostani T, Fan Y, Kolbe T, Breitbach M, Addicks K, Welz A, Brem G, Hescheler J, Aszodi A, Costell M, Bloch W, Fleischmann BK
Cardiovascular research 2008 Dec 1;80(3):435-44
Cardiovascular research 2008 Dec 1;80(3):435-44
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- R1041B Biotin conjugated Collagen IV antibody (Lot 25440, 1/400, 45 min RT) showed strong staining in FFPE sections of Human kidney (Left) with strong Red staining observed in glomeruli and liver (Right) with strong staining in sinusoids. Fixation: formalin fixed paraffin embedded. Antigen retrieval: steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Primary antibody: Collagen IV antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen IV is extracellular. Staining: Collagen IV as precipitated red signal with hematoxylin purple nuclear counterstain.
