HPA062236

antibody from Atlas Antibodies

Targeting: LBR DHCR14B, TDRD18

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA062236

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA062236, RRID:AB_2684721

Product name

Anti-LBR

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

EARREVEVKLTPLILKPFGNSISRYNGEPEHIERN
DAPHKNTQEKFNLSQESSYIATQYSLRPRREEVKL
KEIDSKEEKYVAKELAVRTF


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in HL-60 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LBR antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines HEK293 and MCF-7 using Anti-LBR antibody. Corresponding LBR RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line RH-30 shows localization to nuclear membrane.

Sample type

HUMAN