PA5-85747

antibody from Invitrogen Antibodies

Targeting: CAMK2B CAM2, CAMK2, CAMKB, CaMKIIβ

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-85747

Provider

Invitrogen Antibodies

Product name

CaMKII beta Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Keep as concentrated solution.

Concentration

0.24 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CaMKII beta in various tissue extracts using CaMKII beta polyclonal antibody (Product # PA5-85747) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CaMKII beta was performed by separating 30 µg of human tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CaMKII beta Polyclonal Antibody (Product # PA5-85747) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CaMKII beta Polyclonal Antibody (Product # PA5-85747). Various tissue extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII beta Polyclonal Antibody (Product # PA5-85747) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CaMKII beta Polyclonal Antibody detects CaMKII beta protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: CaMKII beta protein stained by CaMKII beta Polyclonal Antibody (Product # PA5-85747) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta-3 Tubulin Polyclonal Antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CaMKII beta Polyclonal Antibody detects CAMK2B protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. CaMKII beta Polyclonal Antibody (Product # PA5-85747) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CaMKII beta Polyclonal Antibody detects CaMKII beta protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: CaMKII beta protein stained by CaMKII beta Polyclonal Antibody (Product # PA5-85747) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .