IC1419P

antibody from R&D Systems

Targeting: BGLAP OCN

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC1419P

Provider

R&D Systems

Product name

Human Osteocalcin PE-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Osteocalcin in direct ELISAs.

Reactivity

Human

Host

Mouse

Antigen sequence

P02818


Isotype

IgG

Antibody clone number

190125

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

References

Submitted references

Cumulative Rheumatic Inflammation Modulates the Bone-Vascular Axis and Risk of Coronary Calcification.

Chan YH, Ngai MC, Chen Y, Wu MZ, Yu YJ, Zhen Z, Lai K, Cheung T, Ho LM, Chung HY, Lau CS, Tse HF, Yiu KH
Journal of the American Heart Association 2019 Jun 4;8(11):e011540

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Type 2 diabetes affects bone cells precursors and bone turnover.

Sassi F, Buondonno I, Luppi C, Spertino E, Stratta E, Di Stefano M, Ravazzoli M, Isaia G, Trento M, Passera P, Porta M, Isaia GC, D'Amelio P
BMC endocrine disorders 2018 Aug 8;18(1):55

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Age, gender, and percentage of circulating osteoprogenitor (COP) cells: The COP Study.

Gunawardene P, Al Saedi A, Singh L, Bermeo S, Vogrin S, Phu S, Suriyaarachchi P, Pignolo RJ, Duque G
Experimental gerontology 2017 Oct 1;96:68-72

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NZ-GMP Approved Serum Improve hDPSC Osteogenic Commitment and Increase Angiogenic Factor Expression.

Spina A, Montella R, Liccardo D, De Rosa A, Laino L, Mitsiadis TA, La Noce M
Frontiers in physiology 2016;7:354

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Bone disease in newly diagnosed lupus nephritis patients.

Resende AL, dos Reis LM, Dias CB, Custódio MR, Jorgetti V, Woronik V
PloS one 2014;9(9):e106728

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Histone deacetylase inhibition with valproic acid downregulates osteocalcin gene expression in human dental pulp stem cells and osteoblasts: evidence for HDAC2 involvement.

Paino F, La Noce M, Tirino V, Naddeo P, Desiderio V, Pirozzi G, De Rosa A, Laino L, Altucci L, Papaccio G
Stem cells (Dayton, Ohio) 2014 Jan;32(1):279-89

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Osteocalcin in Differentiated Human Mesemchymal Progenitor Cells by Flow Cytometry. Differentiated human mesemchymal progenitor cells were stained with Mouse Anti-Human Osteocalcin PE-conjugated Monoclonal Antibody (Catalog # IC1419P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Osteocalcin in Saos-2 Human Cell Line by Flow Cytometry. Saos-2 human osteosarcoma cell line was stained with Mouse Anti-Human Osteocalcin PE-conjugated Monoclonal Antibody (Catalog # IC1419P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.