RQ6417

antibody from NSJ Bioreagents

Targeting: CYP51A1 CP51, CYP51, CYPL1, LDM, P450-14DM, P450L1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ6417

Provider

NSJ Bioreagents

Product name

Lanosterol 14-alpha demethylase Antibody / CYP51A1

Antibody type

Polyclonal

Description

Purified

Reactivity

Human, Mouse, Simian

Host

Rabbit

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Lanosterol 14-alpha demethylase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) monkey COS-7, 2) human A431, 3) human K562, 4) mouse liver and 5) mouse kidney tissue lysate with Lanosterol 14-alpha demethylase antibody. Predicted molecular weight ~57 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human Caco-2 cells with Lanosterol 14-alpha demethylase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HepG2 cells with Lanosterol 14-alpha demethylase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Lanosterol 14-alpha demethylase antibody.