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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- ARG54002 - Provider product page
- Provider
- Arigo
- Product name
- anti-SOX-2 antibody
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant mouse Sox2 protein fragments expressed in E.coli
- Description
- Affinity purified
- Reactivity
- Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2A10-F11-F12
- Vial size
- 100 µl
- Concentration
- Lot dependent
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: Sox2 in mES cell lysates stained with Sox2 antibody [2A10-F11-F12] (ARG54002) (diluted in 1: 1000). Predicted band size: 35KDa, Observed band size: 35KDa. Kindly provided by Dr. Qintong Li at the College of Life Sciences, Sichuan University.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: Sox2 in F9 cell lysates stained with Sox2 antibody [2A10-F11-F12] (ARG54002) (diluted in 1: 1000). Predicted band size: 35KDa. Observed band size: 35KDa.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: COS7 cells stained with Sox2 antibody [2A10-F11-F12] (ARG54002) (diluted in 1:150).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: F9 cells stained with Sox2 antibody [2A10-F11-F12] (ARG54002) (red, 1/100), followed by FITC-conjugated goat mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.
