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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB3876 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3876, RRID:AB_1580915
- Product name
- SOX2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of SOX2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors.
Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers.
Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4.
Looijenga LH, Stoop H, de Leeuw HP, de Gouveia Brazao CA, Gillis AJ, van Roozendaal KE, van Zoelen EJ, Weber RF, Wolffenbuttel KP, van Dekken H, Honecker F, Bokemeyer C, Perlman EJ, Schneider DT, Kononen J, Sauter G, Oosterhuis JW
Cancer research 2003 May 1;63(9):2244-50
Cancer research 2003 May 1;63(9):2244-50
Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers.
Reményi A, Lins K, Nissen LJ, Reinbold R, Schöler HR, Wilmanns M
Genes & development 2003 Aug 15;17(16):2048-59
Genes & development 2003 Aug 15;17(16):2048-59
Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4.
Nichols J, Zevnik B, Anastassiadis K, Niwa H, Klewe-Nebenius D, Chambers I, Schöler H, Smith A
Cell 1998 Oct 30;95(3):379-91
Cell 1998 Oct 30;95(3):379-91
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of SOX2 (arrow) using rabbit SOX2 polyclonal antibody (Cat # PAB3876). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of SOX2 polyclonal antibody (Cat # PAB3876) in 293 cell line lysates (35 ug/lane). SOX2 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of SOX2 polyclonal antibody (Cat # PAB3876) in HeLa cells. 0.025 mg/mL primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 polyclonal antibody (Cat # PAB3876) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NCI-H292 cells using SOX2 polyclonal antibody (Cat # PAB3876)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
