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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP11920PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP11920PU-N, RRID:AB_1771245
- Product name
- anti SOX2 (N-term)
- Antibody type
- Polyclonal
- Antigen
- This SOX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97~126 amino selected from the N-terminal region of human SOX2.
- Reactivity
- Human, Mouse, Zebrafish
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of SOX2 Antibody (N-term) in 293 cell line lysates (35 µg/lane). SOX2 (arrow) was detected using the purified Pab.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of SOX2 (arrow) using rabbit polyclonal SOX2 Antibody (N-term) (AP11920PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of SOX2 Antibody (N-term) (AP11920PU-N) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 antibody (N-term) (AP11920PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of NCI-H292 cells using SOX2 Antibody (N-term) (AP11920PU-N)(Bottom Histogram) compared to a Negative Control cell (Top Histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.
