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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00000095-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000095-M01, RRID:AB_425286
- Product name
- ACY1 monoclonal antibody (M01), clone 4F1-B7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant ACY1.
- Antigen sequence
MTSKGPAEEHPSVTLFRQYLRIRTVQPKPDYGAAV
AFFEETARQLGLGCQKVEVAPGYVVTVLTWPGTNP
TLSSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGY
IYARGAQDVKCVSIQYLEAVRRLKVEGHRFPRTIH
MTFVPDEEVGGHQGMELFVQRPEFHALRAGFALDE
GIANPTDAFTVFYSERSPWWVRVTSTGRPGHASRF
MEDTAAEKLHKVVNSILAFREKEWQRLQSNPHLKE
GSVTSVNLTKLEGGVAYNVIPATMSASFDFRVAPD
VDFKAFEEQLQSWCQAAGEGVTLEFAQKWMHPQVT
PTDDSNPWWAAFSRVCKDMNLTLEPEIMPAATDNR
YIRAVGVPALGFSPMNRTPVLLHDHDERLHEAVFL
RGVDIYTRLLPALASVPALPSDS- Isotype
- IgG
- Antibody clone number
- 4F1-B7
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Profiling of antibody production against xenograft-released proteins by protein microarrays discovers prostate cancer markers.
Jansen FH, van Rijswijk A, Teubel W, van Weerden WM, Reneman S, van den Bemd GJ, Roobol MJ, Bangma CH, Staal FJ, Jenster G
Journal of proteome research 2012 Feb 3;11(2):728-35
Journal of proteome research 2012 Feb 3;11(2):728-35
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of ACY1 expression in transfected 293T cell line by ACY1 monoclonal antibody (M01), clone 4F1-B7.Lane 1: ACY1 transfected lysate(45.9 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to ACY1 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of ACY1 transfected lysate using anti-ACY1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ACY1 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to ACY1 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
