666904

antibody from BioLegend

Targeting: MUC16 CA125, FLJ14303

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

666904

Provider

BioLegend

Proper citation

BioLegend Cat#666904, RRID:AB_2629540

Product name

Purified anti-CA125

Antibody type

Monoclonal

Antigen

Endogenous protein

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

618F

Vial size

100 µg

Concentration

0.5 μ/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysates (15 µg protein) from HeLa, MCF-7 and U87-MG were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-CA125 (clone 618F) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-GAPDH (clone Poly6314) antibody was used as a loading control.

Conjugate

Unconjugated