Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- V3494 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- MUC16 Antibody / CA125
- Antibody type
- Monoclonal
- Description
- Protein G affinity chromatography
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 5E11.
- Vial size
- 100 µg
- Concentration
- 0.2 mg/ml
- Storage
- Store the MUC16 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Mucins MUC16 and MUC1 are major carriers of SLe(a) and SLe(x) in borderline and malignant serous ovarian tumors.
A novel monoclonal antibody to a defined peptide epitope in MUC16.
Detection of glyco-mucin profiles improves specificity of MUC16 and MUC1 biomarkers in ovarian serous tumours.
Ricardo S, Marcos-Silva L, Valente C, Coelho R, Gomes R, David L
Virchows Archiv : an international journal of pathology 2016 Jun;468(6):715-22
Virchows Archiv : an international journal of pathology 2016 Jun;468(6):715-22
A novel monoclonal antibody to a defined peptide epitope in MUC16.
Marcos-Silva L, Ricardo S, Chen K, Blixt O, Arigi E, Pereira D, Høgdall E, Mandel U, Bennett EP, Vakhrushev SY, David L, Clausen H
Glycobiology 2015 Nov;25(11):1172-82
Glycobiology 2015 Nov;25(11):1172-82
Detection of glyco-mucin profiles improves specificity of MUC16 and MUC1 biomarkers in ovarian serous tumours.
Ricardo S, Marcos-Silva L, Pereira D, Pinto R, Almeida R, Söderberg O, Mandel U, Clausen H, Felix A, Lunet N, David L
Molecular oncology 2015 Feb;9(2):503-12
Molecular oncology 2015 Feb;9(2):503-12
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Supportive validation
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- NSJ Bioreagents (provider)
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- Experimental details
- IHC testing of FFPE human ovarian carcinoma with MUC16 antibody (clone 5E11). Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.