Explore
Validate
Learn
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-12425 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CA125 Monoclonal Antibody (M11)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA5-12429 targets Cytokeratin 5/6 in IHC (P) applications and shows reactivity with Human samples. The MA5-12429 immunogen is isolated cytokeratin 5.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- M11
- Vial size
- 500 µL
- Concentration
- Lot Dependent
- Storage
- 4° C
Submitted references A patient with a noninvasive mucinous ovarian borderline tumor presenting with late pleural metastases.
Gel-forming and cell-associated mucins: preparation for structural and functional studies.
A metalloproteinase secreted by Streptococcus pneumoniae removes membrane mucin MUC16 from the epithelial glycocalyx barrier.
Interfacial interaction between transmembrane ocular mucins and adhesive polymers and dendrimers analyzed by surface plasmon resonance.
Notch signaling modulates MUC16 biosynthesis in an in vitro model of human corneal and conjunctival epithelial cell differentiation.
Simons M, Nagtegaal ID, Overbeek LI, Flucke U, Massuger LF, Bulten J
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 2015 Mar;34(2):143-50
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 2015 Mar;34(2):143-50
Gel-forming and cell-associated mucins: preparation for structural and functional studies.
Davies JR, Wickström C, Thornton DJ
Methods in molecular biology (Clifton, N.J.) 2012;842:27-47
Methods in molecular biology (Clifton, N.J.) 2012;842:27-47
A metalloproteinase secreted by Streptococcus pneumoniae removes membrane mucin MUC16 from the epithelial glycocalyx barrier.
Govindarajan B, Menon BB, Spurr-Michaud S, Rastogi K, Gilmore MS, Argüeso P, Gipson IK
PloS one 2012;7(3):e32418
PloS one 2012;7(3):e32418
Interfacial interaction between transmembrane ocular mucins and adhesive polymers and dendrimers analyzed by surface plasmon resonance.
Bravo-Osuna I, Noiray M, Briand E, Woodward AM, Argüeso P, Molina Martínez IT, Herrero-Vanrell R, Ponchel G
Pharmaceutical research 2012 Aug;29(8):2329-40
Pharmaceutical research 2012 Aug;29(8):2329-40
Notch signaling modulates MUC16 biosynthesis in an in vitro model of human corneal and conjunctival epithelial cell differentiation.
Xiong L, Woodward AM, Argüeso P
Investigative ophthalmology & visual science 2011 Jul 29;52(8):5641-6
Investigative ophthalmology & visual science 2011 Jul 29;52(8):5641-6
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of CA 125 was performed using 70% confluent log phase SKOV3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CA 125 (M11) Mouse Monoclonal Antibody (Product # MA5-12425) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175)a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with CA125 using peroxidase-conjugate and AEC chromogen. Note membrane and cytoplasmic staining of tumor cells.
