Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunohistochemistry [3]
- Blocking/Neutralizing [2]
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Validation data
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- Product number
- AF-557-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal CNTF Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects rat CNTF in direct ELISAs and Western blots. Detects mouse and rat CNTF in immunohistochemistry. In direct ELISAs and Western blots, less than 15% cross-reactivity with recombinant human CNTF is observed.
- Reactivity
- Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references The Rare IL22RA2 Signal Peptide Coding Variant rs28385692 Decreases Secretion of IL-22BP Isoform-1, -2 and -3 and Is Associated with Risk for Multiple Sclerosis.
Therapeutic Potential of AAV1-Rheb(S16H) Transduction Against Alzheimer's Disease.
Opposite effects of a high-fat diet and calorie restriction on ciliary neurotrophic factor signaling in the mouse hypothalamus.
AAV-mediated expression of CNTF promotes long-term survival and regeneration of adult rat retinal ganglion cells.
Cardiotrophin-1 in choroid plexus and the cerebrospinal fluid circulatory system.
Gómez-Fernández P, Lopez de Lapuente Portilla A, Astobiza I, Mena J, Urtasun A, Altmann V, Matesanz F, Otaegui D, Urcelay E, Antigüedad A, Malhotra S, Montalban X, Castillo-Triviño T, Espino-Paisán L, Aktas O, Buttmann M, Chan A, Fontaine B, Gourraud PA, Hecker M, Hoffjan S, Kubisch C, Kümpfel T, Luessi F, Zettl UK, Zipp F, Alloza I, Comabella M, Lill CM, Vandenbroeck K
Cells 2020 Jan 10;9(1)
Cells 2020 Jan 10;9(1)
Therapeutic Potential of AAV1-Rheb(S16H) Transduction Against Alzheimer's Disease.
Moon GJ, Kim S, Jeon MT, Lee KJ, Jang IS, Nakamura M, Kim SR
Journal of clinical medicine 2019 Nov 22;8(12)
Journal of clinical medicine 2019 Nov 22;8(12)
Opposite effects of a high-fat diet and calorie restriction on ciliary neurotrophic factor signaling in the mouse hypothalamus.
Severi I, Perugini J, Mondini E, Smorlesi A, Frontini A, Cinti S, Giordano A
Frontiers in neuroscience 2013;7:263
Frontiers in neuroscience 2013;7:263
AAV-mediated expression of CNTF promotes long-term survival and regeneration of adult rat retinal ganglion cells.
Leaver SG, Cui Q, Plant GW, Arulpragasam A, Hisheh S, Verhaagen J, Harvey AR
Gene therapy 2006 Sep;13(18):1328-41
Gene therapy 2006 Sep;13(18):1328-41
Cardiotrophin-1 in choroid plexus and the cerebrospinal fluid circulatory system.
Gard AL, Gavin E, Solodushko V, Pennica D
Neuroscience 2004;127(1):43-52
Neuroscience 2004;127(1):43-52
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- CNTF in Rat Cerebellum. CNTF in Rat Cerebellum.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- CNTF in Mouse Cerebellum. CNTF in Mouse Cerebellum.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- CNTF in Mouse Brain. CNTF in Mouse Brain.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by CNTF and Neutralization by Rat CNTF Antibody. Recom-binant Rat CNTF (Catalog # 557-NT) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat CNTF (200 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA). The ND50 is typ-ically 4-8 µg/mL.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by CNTF and Neutralization by Rat CNTF Antibody. Cell Proliferation Induced by CNTF and Neutralization by Rat CNTF Antibody.