Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 362707 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#362707, RRID:AB_2564401
- Product name
- APC anti-human/mouse CD3ε (activation epitope)
- Antibody type
- Monoclonal
- Antigen
- Purified denatured CD3 proteins from human thymus
- Description
- The CD3ε chain of the CD3-TCR complex is a type I membrane protein that undergoes conformational change following ligation of the TCR-CD3 complex. This conformational change leads to exposure of the proline rich sequence in CD3ε and results in recruitment of Nck. It is expressed by all mature T cells and forms a heteromeric complex with CD3 α/β or γ/δ and ζ chains. It is important in T cell development, and deficiency of this gene has been associated with severe immunodeficiency.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Red dye
- Antibody clone number
- APA1/1
- Vial size
- 25 tests
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Human peripheral blood lymphocytes were fixed and permeabilized with 70% ethanol, followed by intracellular staining with CD3e (clone APA1/1) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
- Conjugate
- Red dye