NBP1-49045

antibody from Novus Biologicals

Targeting: CD8A CD8

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

NBP1-49045

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-49045, RRID:AB_10011327

Product name

Rat Monoclonal CD8 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified.

Reactivity

Mouse, Rat

Host

Rat

Isotype

IgG

Vial size

0.5 mg

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

CXCL14 suppresses human papillomavirus-associated head and neck cancer through antigen-specific CD8(+) T-cell responses by upregulating MHC-I expression.

Westrich JA, Vermeer DW, Silva A, Bonney S, Berger JN, Cicchini L, Greer RO, Song JI, Raben D, Slansky JE, Lee JH, Spanos WC, Pyeon D
Oncogene 2019 Nov;38(46):7166-7180

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Acid sensing ion channel 2: A new potential player in the pathophysiology of multiple sclerosis.

Fazia T, Pastorino R, Notartomaso S, Busceti C, Imbriglio T, Cannella M, Gentilini D, Morani G, Ticca A, Bitti P, Berzuini C, Dalmay T, Battaglia G, Bernardinelli L
The European journal of neuroscience 2019 May;49(10):1233-1243

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Chemotherapy induces enrichment of CD47⁺/CD73⁺/PDL1⁺ immune evasive triple-negative breast cancer cells.

Samanta D, Park Y, Ni X, Li H, Zahnow CA, Gabrielson E, Pan F, Semenza GL
Proceedings of the National Academy of Sciences of the United States of America 2018 Feb 6;115(6):E1239-E1248

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Anti-Jagged Immunotherapy Inhibits MDSCs and Overcomes Tumor-Induced Tolerance.

Sierra RA, Trillo-Tinoco J, Mohamed E, Yu L, Achyut BR, Arbab A, Bradford JW, Osborne BA, Miele L, Rodriguez PC
Cancer research 2017 Oct 15;77(20):5628-5638

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Blockade of adenosine A2A receptor enhances CD8⁺ T cells response and decreases regulatory T cells in head and neck squamous cell carcinoma.

Ma SR, Deng WW, Liu JF, Mao L, Yu GT, Bu LL, Kulkarni AB, Zhang WF, Sun ZJ
Molecular cancer 2017 Jun 7;16(1):99

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Fibroblasts drive an immunosuppressive and growth-promoting microenvironment in breast cancer via secretion of Chitinase 3-like 1.

Cohen N, Shani O, Raz Y, Sharon Y, Hoffman D, Abramovitz L, Erez N
Oncogene 2017 Aug;36(31):4457-4468

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In Vivo Magnetic Resonance Imaging of CD8+ T Lymphocytes Recruiting to Glioblastoma in Mice.

Li A, Wu Y, Tang F, Li W, Feng X, Yao Z
Cancer biotherapy & radiopharmaceuticals 2016 Nov;31(9):317-323

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Cinnabarinic acid, an endogenous agonist of type-4 metabotropic glutamate receptor, suppresses experimental autoimmune encephalomyelitis in mice.

Fazio F, Zappulla C, Notartomaso S, Busceti C, Bessede A, Scarselli P, Vacca C, Gargaro M, Volpi C, Allegrucci M, Lionetto L, Simmaco M, Belladonna ML, Nicoletti F, Fallarino F
Neuropharmacology 2014 Jun;81:237-43

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Permanent protection of PLG scaffold transplanted allogeneic islet grafts in diabetic mice treated with ECDI-fixed donor splenocyte infusions.

Kheradmand T, Wang S, Gibly RF, Zhang X, Holland S, Tasch J, Graham JG, Kaufman DB, Miller SD, Shea LD, Luo X
Biomaterials 2011 Jul;32(20):4517-24

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Effects of TCDD on the fate of naive dendritic cells.

Bankoti J, Burnett A, Navarro S, Miller AK, Rase B, Shepherd DM
Toxicological sciences : an official journal of the Society of Toxicology 2010 Jun;115(2):422-34

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Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression.

McLellan AD, Kapp M, Eggert A, Linden C, Bommhardt U, Bröcker EB, Kämmerer U, Kämpgen E
Blood 2002 Mar 15;99(6):2084-93

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Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression.

McLellan AD, Kapp M, Eggert A, Linden C, Bommhardt U, Bröcker EB, Kämmerer U, Kämpgen E
Blood 2002 Mar 15;99(6):2084-93

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CD4 and CD8 regulate interleukin 2 responses of T cells.

Takahashi K, Nakata M, Tanaka T, Adachi H, Nakauchi H, Yagita H, Okumura K
Proceedings of the National Academy of Sciences of the United States of America 1992 Jun 15;89(12):5557-61

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CD4 and CD8 regulate interleukin 2 responses of T cells.

Takahashi K, Nakata M, Tanaka T, Adachi H, Nakauchi H, Yagita H, Okumura K
Proceedings of the National Academy of Sciences of the United States of America 1992 Jun 15;89(12):5557-61

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Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens.

Ledbetter JA, Herzenberg LA
Immunological reviews 1979;47:63-90

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Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens.

Ledbetter JA, Herzenberg LA
Immunological reviews 1979;47:63-90

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CD8 alpha Antibody (53-6.7) [NBP1-49045] - CD8 alpha expression in mouse spleen tissue using anti-CD8 alpha antibody. Image from verified customer review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of mosue spleen sections. CD8+ lymphocytes are marked by brown labeling of the cell surface.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of CD8+ T cells in allogeneic skin grafted onto a mouse.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of frozen spleen sections. Blocking: 1 hour at 25C with serum at 5% concentration .

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - C57BL/6 mouse splenocytes were stained with biotinylated CD8 (clone 53-6.7) (filled histogram) or rat IgG2a, k isotype control (open histogram), followed by Sav-PE.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - C57BL/6 mouse splenocytes were stained with purified CD8 (clone 53-6.7) (filled histogram) or rat IgG2a, k isotype control (open histogram), followed by anti-rat IgG FITC.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of fixed murine splenocytes by multiple staining.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Staining of peripheral blood leukocytes.