AF4297

antibody from R&D Systems

Targeting: CD8A CD8

Western blot (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

AF4297

Provider

R&D Systems

Product name

Mouse MGL1/2 (CD301a/b) Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse MGL1 and MGL2 in direct ELISAs and Western blots.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

AAH14811


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice.

Sung SJ, Ge Y, Dai C, Wang H, Fu SM, Sharma R, Hahn YS, Yu J, Le TH, Okusa MD, Bolton WK, Lawler JR
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 1;198(7):2589-2601

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T-cell recruitment and Th1 polarization in adipose tissue during diet-induced obesity in C57BL/6 mice.

Strissel KJ, DeFuria J, Shaul ME, Bennett G, Greenberg AS, Obin MS
Obesity (Silver Spring, Md.) 2010 Oct;18(10):1918-25

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of MGL1/2 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse MGL1/2 (CD301a/b) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4297, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).