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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB2496 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2496, RRID:AB_1572366
- Product name
- CD8A polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of CD8A.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Gly111Ser mutation in CD8A gene causing CD8 immunodeficiency is found in Spanish Gypsies.
CD8+ T cells contribute to macrophage accumulation and airspace enlargement following repeated irritant exposure.
CD8+FOXP3+T cells from renal transplant recipients in quiescence induce immunoglobulin-like transcripts-3 and -4 on dendritic cells from their respective donors.
Mancebo E, Moreno-Pelayo MA, Mencía A, de la Calle-Martín O, Allende LM, Sivadorai P, Kalaydjieva L, Bertranpetit J, Coto E, Calleja-Antolín S, Ruiz-Contreras J, Paz-Artal E
Molecular immunology 2008 Jan;45(2):479-84
Molecular immunology 2008 Jan;45(2):479-84
CD8+ T cells contribute to macrophage accumulation and airspace enlargement following repeated irritant exposure.
Borchers MT, Wesselkamper SC, Harris NL, Deshmukh H, Beckman E, Vitucci M, Tichelaar JW, Leikauf GD
Experimental and molecular pathology 2007 Dec;83(3):301-10
Experimental and molecular pathology 2007 Dec;83(3):301-10
CD8+FOXP3+T cells from renal transplant recipients in quiescence induce immunoglobulin-like transcripts-3 and -4 on dendritic cells from their respective donors.
Zhou H, Wang ZD, Zhu X, You Y, Zou P
Transplantation proceedings 2007 Dec;39(10):3065-7
Transplantation proceedings 2007 Dec;39(10):3065-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CD8A (arrow) using rabbit CD8A polyclonal antibody (Cat # PAB2496). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD8A gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD8A polyclonal antibody (Cat # PAB2496) at a 1 : 50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NCI-H292 cells using CD8A polyclonal antibody (Cat # PAB2496)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
