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Flow cytometry
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [13]
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- Validations
- Flow cytometry [1]
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- Product number
- 16-0086-81 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD8a Monoclonal Antibody (OKT8 (OKT-8)), Functional Grade, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The OKT8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alphaalpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells. Preliminary testing indicates that OKT8 and two other mouse anti-human CD8 antibodies (clone RPA-T8, Cat. No.14-0088 and clone HIT8a, Cat. No.14-0089) do not compete with each other for binding to human peripheral blood leukocytes by flow cytometric analysis, suggesting that they do not bind to similar epitopes or block each other by steric hindrance. Applications Reported: This OKT8 (OKT-8) antibody has been reported for use in flow cytometric analysis. Applications Tested: This OKT8 (OKT-8) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OKT8 (OKT-8)
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C
Submitted references Disruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma.
Extensive T cell cross-reactivity between diverse seasonal influenza strains in the ferret model.
Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma.
PD-L1 expression and prognostic impact in glioblastoma.
Increased circulating IL-9-producing CD8+ T cells are associated with eosinophilia and high FeNO in allergic asthmatics.
Structure and Membrane Binding Properties of the Endosomal Tetratricopeptide Repeat (TPR) Domain-containing Sorting Nexins SNX20 and SNX21.
TL1A increases expression of CD25, LFA-1, CD134 and CD154, and induces IL-22 and GM-CSF production from effector CD4 T-cells.
CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.
MHC multimer-guided and cell culture-independent isolation of functional T cell receptors from single cells facilitates TCR identification for immunotherapy.
Mechanistic insight into pertussis toxin and lectin signaling using T cells engineered to express a CD8α/CD3ζ chimeric receptor.
Simian immunodeficiency virus-induced changes in T cell cytokine responses in cynomolgus macaques with latent Mycobacterium tuberculosis infection are associated with timing of reactivation.
Discordance between antibody and T cell responses in recipients of trivalent inactivated influenza vaccine.
The X-linked lymphoproliferative-disease gene product SAP regulates signals induced through the co-receptor SLAM.
Guo X, Jiang H, Shi B, Zhou M, Zhang H, Shi Z, Du G, Luo H, Wu X, Wang Y, Sun R, Li Z
Frontiers in pharmacology 2018;9:1118
Frontiers in pharmacology 2018;9:1118
Extensive T cell cross-reactivity between diverse seasonal influenza strains in the ferret model.
Reber AJ, Music N, Kim JH, Gansebom S, Chen J, York I
Scientific reports 2018 Apr 17;8(1):6112
Scientific reports 2018 Apr 17;8(1):6112
Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma.
Liu Z, Han H, He X, Li S, Wu C, Yu C, Wang S
Oncology letters 2016 Mar;11(3):1829-1834
Oncology letters 2016 Mar;11(3):1829-1834
PD-L1 expression and prognostic impact in glioblastoma.
Nduom EK, Wei J, Yaghi NK, Huang N, Kong LY, Gabrusiewicz K, Ling X, Zhou S, Ivan C, Chen JQ, Burks JK, Fuller GN, Calin GA, Conrad CA, Creasy C, Ritthipichai K, Radvanyi L, Heimberger AB
Neuro-oncology 2016 Feb;18(2):195-205
Neuro-oncology 2016 Feb;18(2):195-205
Increased circulating IL-9-producing CD8+ T cells are associated with eosinophilia and high FeNO in allergic asthmatics.
Wang W, Cheng ZS, Chen YF, Lin YH
Experimental and therapeutic medicine 2016 Dec;12(6):4055-4060
Experimental and therapeutic medicine 2016 Dec;12(6):4055-4060
Structure and Membrane Binding Properties of the Endosomal Tetratricopeptide Repeat (TPR) Domain-containing Sorting Nexins SNX20 and SNX21.
Clairfeuille T, Norwood SJ, Qi X, Teasdale RD, Collins BM
The Journal of biological chemistry 2015 Jun 5;290(23):14504-17
The Journal of biological chemistry 2015 Jun 5;290(23):14504-17
TL1A increases expression of CD25, LFA-1, CD134 and CD154, and induces IL-22 and GM-CSF production from effector CD4 T-cells.
Reichwald K, Jørgensen TZ, Skov S
PloS one 2014;9(8):e105627
PloS one 2014;9(8):e105627
CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.
Zhang W, Nie L, Wang Y, Wang XP, Zhao H, Dongol S, Maharjan S, Cheng L
PloS one 2013;8(6):e66286
PloS one 2013;8(6):e66286
MHC multimer-guided and cell culture-independent isolation of functional T cell receptors from single cells facilitates TCR identification for immunotherapy.
Dössinger G, Bunse M, Bet J, Albrecht J, Paszkiewicz PJ, Weißbrich B, Schiedewitz I, Henkel L, Schiemann M, Neuenhahn M, Uckert W, Busch DH
PloS one 2013;8(4):e61384
PloS one 2013;8(4):e61384
Mechanistic insight into pertussis toxin and lectin signaling using T cells engineered to express a CD8α/CD3ζ chimeric receptor.
Schneider OD, Millen SH, Weiss AA, Miller WE
Biochemistry 2012 May 22;51(20):4126-37
Biochemistry 2012 May 22;51(20):4126-37
Simian immunodeficiency virus-induced changes in T cell cytokine responses in cynomolgus macaques with latent Mycobacterium tuberculosis infection are associated with timing of reactivation.
Mattila JT, Diedrich CR, Lin PL, Phuah J, Flynn JL
Journal of immunology (Baltimore, Md. : 1950) 2011 Mar 15;186(6):3527-37
Journal of immunology (Baltimore, Md. : 1950) 2011 Mar 15;186(6):3527-37
Discordance between antibody and T cell responses in recipients of trivalent inactivated influenza vaccine.
Co MD, Orphin L, Cruz J, Pazoles P, Rothman AL, Ennis FA, Terajima M
Vaccine 2008 Apr 7;26(16):1990-8
Vaccine 2008 Apr 7;26(16):1990-8
The X-linked lymphoproliferative-disease gene product SAP regulates signals induced through the co-receptor SLAM.
Sayos J, Wu C, Morra M, Wang N, Zhang X, Allen D, van Schaik S, Notarangelo L, Geha R, Roncarolo MG, Oettgen H, De Vries JE, Aversa G, Terhorst C
Nature 1998 Oct 1;395(6701):462-9
Nature 1998 Oct 1;395(6701):462-9
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were stained with CD19 Monoclonal Antibody, APC (Product # 17-0199-42) and 0.125 µg Mouse IgG2a kappa Isotype Control (Product # 14-4724-82) (left) or 0.125 µg CD8 Monoclonal Antibody, Functional Grade Purified (right) followed by IgG2a Monoclonal Antibody, PE (Product # 12-4210-82). Cells in the lymphocyte gate were used for analysis.
